1.5.1.10: saccharopine dehydrogenase (NADP+, L-glutamate-forming)
This is an abbreviated version!
For detailed information about saccharopine dehydrogenase (NADP+, L-glutamate-forming), go to the full flat file.
Word Map on EC 1.5.1.10
-
1.5.1.10
-
alpha-aminoadipate
-
auxotrophs
-
magnaporthe
-
grisea
-
homocitrate
-
pipecolic
-
swainsonine
-
homoisocitrate
-
piperideine-6-carboxylic
-
chrysogenum
-
unlinked
-
rossmann
-
penicillium
-
ph-rate
-
endophytic
-
embedding
-
6-dehydrogenase
-
substrate-assisted
-
seven-stranded
-
diaminopimelate
-
oxytropis
-
acid-base
-
leaky
-
all-helical
-
anisotropic
-
l-pipecolate
-
dl-alpha-aminoadipate
-
gene-enzyme
- 1.5.1.10
- alpha-aminoadipate
-
auxotrophs
-
magnaporthe
- grisea
- homocitrate
-
pipecolic
- swainsonine
- homoisocitrate
-
piperideine-6-carboxylic
- chrysogenum
-
unlinked
-
rossmann
-
penicillium
-
ph-rate
-
endophytic
-
embedding
-
6-dehydrogenase
-
substrate-assisted
-
seven-stranded
- diaminopimelate
- oxytropis
-
acid-base
-
leaky
-
all-helical
-
anisotropic
- l-pipecolate
- dl-alpha-aminoadipate
-
gene-enzyme
Reaction
Synonyms
aminoadipate semialdehyde-glutamate reductase, aminoadipic semialdehyde-glutamate reductase, aminoadipic semialdehyde-glutamic reductase, ASS, dehydrogenase, saccharopine (nicotinamide adenine dinucleotide phosphate, glutamate-forming), epsilon-N-(L-glutaryl-2)-L-lysine:NAD+(P) oxidoreductase (L-2-aminoadipate-semialdehyde forming), LKR/SDH, lysine-ketoglutarate reductase/saccharopine dehydrogenase, nSpe-Sdh, saccharopine dehydrogenase, saccharopine dehydrogenase (L-glutamate forming), saccharopine reductase, SDH, spermidine synthase-saccharopine dehydrogenase, SR1
ECTree
Advanced search results
Reaction
Reaction on EC 1.5.1.10 - saccharopine dehydrogenase (NADP+, L-glutamate-forming)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH + H+
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH + H+
-
-
-
-
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH + H+
ordered kinetic mechanism, the reduced dinucleotide substrate binds to enzyme first followed by L-alpha-aminoadipate-delta-semialdehyde, which adds in rapid equilibrium prior to L-glutamate, saccharopine is released prior to NADP+, primary deuterium kinetic isotope effects and solvent deuterium kinetic isotope effects, overview. A conformational change to open the site and release products (in the direction of saccharopine formation) is the rate limiting step. Two groups are involved in the acid-base chemistry of the reaction. An enzyme group with a pKa of 5.6 accepts a proton from the alpha-amine of glutamate to generate the neutral amine that can act as a nucleophile. The alpha-amine of glutamate attacks the carbonyl of the semialdehyde to generate the carbinolamine, which is protonated by a second enzyme group with a pKa of about 7.8-8.0. Ionizable residues that might play a role in the acid-base mechanism of the enzyme are D126, C154 and/or Y99