1.14.99.46: pyrimidine oxygenase
This is an abbreviated version!
For detailed information about pyrimidine oxygenase, go to the full flat file.
Reaction
Synonyms
RutA
ECTree
1.14.99.46 pyrimidine oxygenaseAdvanced search results
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Substrates Products on EC 1.14.99.46 - pyrimidine oxygenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
thymine + FMNH2 + O2
(Z)-2-methylureidoacrylate peracid + FMN
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?
uracil + FMNH2 + O2
(Z)-3-ureidoacrylate peracid + FMN
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?
uracil + FMNH2 + O2
(Z)-3-ureidoacrylate peracid + FMN
RutA directly cleaves the uracil ring between N-3 and C-4 to yield ureidoacrylate
product analysis by both NMR spectroscopy and mass spectrometry, overview
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additional information
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the pyrimidine ring is cleaved directly by adding a pair of oxygen atoms and producing ureidoacrylate peracid. This odd metabolite, a strong and highly toxic oxidizing agent, is reduced spontaneously, albeit slowly, to ureidoacrylate
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additional information
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(Z)-3-ureidoacrylic peracid decomposes nonenzymatically to form primarily (Z)-3-ureidoacrylic acid and uracil, half life of (Z)-3-ureidoacrylic peracid is 3 hours at pH 10.0 at 25°C and much less at lower pH
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additional information
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(Z)-3-ureidoacrylic peracid is not stable and decomposes nonenzymatically to give (Z)-3-ureidoacrylate
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