1.14.19.45: sn-1 oleoyl-lipid 12-desaturase
This is an abbreviated version!
For detailed information about sn-1 oleoyl-lipid 12-desaturase, go to the full flat file.
Word Map on EC 1.14.19.45
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1.14.19.45
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linoleic
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unsaturated
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oleic
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sativa
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oilseed
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polyunsaturated
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camelina
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desaturases
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polyploid
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cereus
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acclimation
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arachis
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monounsaturated
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hypogaea
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crispr-cas9
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fluidity
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hexaploid
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high-oleic
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peanut
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desaturation
- 1.14.19.45
-
linoleic
- unsaturated
-
oleic
- sativa
-
oilseed
-
polyunsaturated
- camelina
-
desaturases
-
polyploid
- cereus
-
acclimation
-
arachis
-
monounsaturated
- hypogaea
-
crispr-cas9
-
fluidity
-
hexaploid
-
high-oleic
- peanut
-
desaturation
Reaction
+ 2 reduced ferredoxin [iron-sulfur] cluster + + 2 H+ = + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
Synonyms
acyl-lipid DELTA12 desaturase, bifunctional DELTA12/DELTA15-fatty acid desaturase, CpFAD2, CsFAD2, DELTA(12) fatty acid desaturase, DELTA12 acyl-lipid desaturase, DELTA12 desaturase, DELTA12 fatty acid desaturase, DELTA12-acyl-lipid desaturase, DELTA12-desaturase, DELTA12-fatty acid desaturase, DELTA5 Des, DELTA5 desaturase, DesA, DesA desaturase, FAD2, FAD2-1, FAD2-2, FAD2-3, More, oleate DELTA12desaturase, RKD12, TcasZ12, YALI0B10153g
ECTree
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Engineering
Engineering on EC 1.14.19.45 - sn-1 oleoyl-lipid 12-desaturase
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additional information
establishment of transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 DELTA12 (desA) acyl-lipid desaturase and Synechococcus vulcanus DELTA9 (desC) acyl-lipid desaturase with or without sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of a constitutive promoter. Reliable increase of linoleic acid portion (C18:2, DELTA9,12) accompanied with decrease of alpha-linolenic acid (C18:3, DELTA9,12,15) relative amount is detected for plants expressing hybrid desA::licBM3 gene. No reliable changes are detected in fatty acid profiles and unsaturation index of plants transformed with DELTA9 desaturase gene desC::licBM3 lacking signals of intracellular targeting while expression of this gene with Arabidopsis thaliana Rubisco small subunit transit peptide sequence caused growth of C18:3 alpha-linolenic acid part simultaneously with reduction of C18:2 linoleic acid part, as well as increase of unsaturation index. No changes in relative amount of DELTA9monounsaturated fatty acids were observed in any of studied lines. All plants expressing desaturase genes exhibited enhanced levels of superoxide dismutase (SOD) activity after cold treatment in contrast to control lines with suppressed SOD activity after cold treatment
additional information
deletion of the entire C-terminal histidine-rich insert of TcasZ12 results in almost total abolition of activity. However, reinstating two histidine residues to the truncated TcasZ12 sequence partially restored DELTA12 desaturase activity