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1.14.19.45: sn-1 oleoyl-lipid 12-desaturase

This is an abbreviated version!
For detailed information about sn-1 oleoyl-lipid 12-desaturase, go to the full flat file.

Word Map on EC 1.14.19.45

Reaction

a 1-oleoyl-2-acyl-[glycerolipid]
+ 2 reduced ferredoxin [iron-sulfur] cluster +
O2
 + 2 H+ =
a 1-linoleoyl-2-acyl-[glycerolipid]
 + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O

Synonyms

acyl-lipid DELTA12 desaturase, bifunctional DELTA12/DELTA15-fatty acid desaturase, CpFAD2, CsFAD2, DELTA(12) fatty acid desaturase, DELTA12 acyl-lipid desaturase, DELTA12 desaturase, DELTA12 fatty acid desaturase, DELTA12-acyl-lipid desaturase, DELTA12-desaturase, DELTA12-fatty acid desaturase, DELTA5 Des, DELTA5 desaturase, DesA, DesA desaturase, FAD2, FAD2-1, FAD2-2, FAD2-3, More, oleate DELTA12desaturase, RKD12, TcasZ12, YALI0B10153g

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.45 sn-1 oleoyl-lipid 12-desaturase

Cloned

Cloned on EC 1.14.19.45 - sn-1 oleoyl-lipid 12-desaturase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Bacillus subtilis
-
expressed in Solanum tuberosum cultivar Desnitsa
-
gene CsFAD2, variant FAD2-1, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview
gene CsFAD2, variant FAD2-2, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview
gene CsFAD2, variant FAD2-3, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A . The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview
gene FAD2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression in Saccharomyces cerevisiae strain BY4741 supplemented with linoleic acid (DELTA9,DELTA12-18:2) and hexadecadienoic acid (DELTA9,DELTA12-16:2) leads to accumulation of DELTA15-PUFAs, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3) results in production of a range of DELTA12- and DELTA15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (DELTA9,DELTA12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (DELTA9,DELTA12,DELTA15-16:3). Ricinoleic acid (12-hydroxy-9-octadecenoic acid) is an additional product of CpFad2 identified by GC/MS analysis
gene RKD12, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae
heterologous expression in Saccharomyces cerevisiae. Plasmid pYES2-egFAD2 is transformed into Saccharomyces cerevisiae strain INVSc1 using lithium acetate method for expression under the induction of galactose
overexpression in Saccharomyces cerevisiae
recombinant expression of hybrid gene of Synechococcus vulcanus DELTA12 (desA) acyl–lipid desaturase in Nicotiana tabacum cv. Wisconsin, as chimeric enzyme DesA-LicBM3 with reporter thermostable lichenase of Clostridium thermocellum, expression with or without the sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of the 35S CaMV constitutive promoter using the Agrobacterium tumefaciens strain GV3101-mediated transformation method, measurement of recombinant lichenase activity. Plants expressing RTP::desA::licBM3 gene coding for DELTA12 desaturase with the transit peptide for transport into chloroplasts causes a reliable increase of DELTA9,12-18:2 fatty acids and a decrease of DELTA9,12,15-18:3 fatty acids
RKD12 is further transformed into Saccharomyces cerevisiae for functional characterization
Solanum tuberosum L., cv. Desnitsa plants of wild type are transformed with desA gene of DELTA12-acyl-lipid desaturase from Synechocystis sp. PCC 6803. The transformed plants differ from the wild-type by elevated polyunsaturated fatty acids content in membrane lipids and greater resistance to oxidative stress under hypothermia
the introduction of the desA gene into potato plants promotes rapid growth as compared to the control plants, which, in turn, is accompanied by changes in the accumulation of pigments and phenolic compounds. The transformed plants show faster growth and faster ontogenesis as compared to controls, which is accompanied with changes in the accumulation of photosynthetic pigments (chlorophylls a and b, carotenoids) and phenolic compounds, including flavonoids in the leaves. These characteristics are pronounced to a high degree in Line II plants with high expression rates of the desA gene, whereas Line I plants (moderate expression rate) are similar to control plants in many parameters
transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 DELTA12 (desA) acyl-lipid desaturase and Synechococcus vulcanus DELTA9 (desC) acyl-lipid desaturase with or without sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of a constitutive promoter are established