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1.14.13.64: 4-hydroxybenzoate 1-hydroxylase

This is an abbreviated version!
For detailed information about 4-hydroxybenzoate 1-hydroxylase, go to the full flat file.

Word Map on EC 1.14.13.64

Reaction

4-hydroxybenzoate
+
NAD(P)H
+
H+
+
O2
=
hydroquinone
+
NAD(P)+
+
H2O
+
CO2

Synonyms

4-hydroxybenzoate 1-hydroxylase, 4-hydroxybenzoate 1-hydroxylase (decarboxylating), 4-hydroxybenzoate 1-monooxygenase, 4HB1H, Cp_4HB1H, FAD-dependent 4-hydroxybenzoate hydroxylase, MNX1, Mnx1p, Oxygenase, 4-hydroxybenzoate 1-mono, PHBH, V1H, vanillate 1-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.64 4-hydroxybenzoate 1-hydroxylase

Purification

Purification on EC 1.14.13.64 - 4-hydroxybenzoate 1-hydroxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain TOP10 by nickel affinity chromatography, dialysis, ion exchange chromatography, and gel filtration
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