1.14.13.64: 4-hydroxybenzoate 1-hydroxylase

This is an abbreviated version!
For detailed information about 4-hydroxybenzoate 1-hydroxylase, go to the full flat file.

Reaction

Synonyms

4-hydroxybenzoate 1-hydroxylase, 4-hydroxybenzoate 1-hydroxylase (decarboxylating), 4-hydroxybenzoate 1-monooxygenase, 4HB1H, Cp_4HB1H, FAD-dependent 4-hydroxybenzoate hydroxylase, MNX1, Mnx1p, Oxygenase, 4-hydroxybenzoate 1-mono, PHBH, V1H, vanillate 1-hydroxylase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.64 4-hydroxybenzoate 1-hydroxylase

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Results	in table
1	AA Sequence
6	Application
1	CAS Registry Number
3	Cloned(Commentary)
17	Cofactor
10	General Information
7	Inhibitors
3	kcat/KM [mM/s]
18	KM Value [mM]
2	Localization
5	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
34	Organism
2	Pathway
5	pH Optimum
2	Posttranslational Modification
1	Protein Variants
3	Purification (Commentary)
3	Reaction
2	Reaction Type
12	Reference
2	Specific Activity [micromol/min/mg]
70	Substrates and Products (Substrate)
10	Subunits
26	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
17	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 1.14.13.64 - 4-hydroxybenzoate 1-hydroxylase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

2,3,5,6-Tetrafluoro-4-hydroxybenzoate + NADH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

2,3,5,6-Tetrafluoro-4-hydroxybenzoate + NADPH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

2,4-dihydroxybenzoate + NAD(P)H + H+ + O2

benzene-1,2,4-triol + NAD(P)+ + H2O + CO2

2 entries

2,4-Dihydroxybenzoate + NADH + O2

1,2,4-Trihydroxybenzene + NAD+ + CO2

2 entries

2,4-dihydroxybenzoate + NADPH + O2

1,2,4-trihydroxybenzene + NADP+ + CO2

Candida parapsilosis

-

-

7428

-

7428

?

2,5-Difluoro-4-hydroxybenzoate + NADH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

2,5-Difluoro-4-hydroxybenzoate + NADPH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

2,6-Difluoro-4-hydroxybenzoate + NADH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

2,6-Difluoro-4-hydroxybenzoate + NADPH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

2-chloro-4-hydroxybenzoate + NADH + O2

?

2 entries

2-Chloro-4-hydroxybenzoate + NADPH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

2-fluoro-4-hydroxybenzoate + NADH + O2

2-fluoro-1,4-dihydroxybenzene + NAD+ + CO2

2 entries

2-fluoro-4-hydroxybenzoate + NADPH + O2

2-fluoro-1,4-dihydroxybenzene + NADP+ + CO2

Candida parapsilosis

-

-

7428

-

7428

?

3,4-dihydroxybenzoate + NAD(P)H + H+ + O2

benzene-1,2,4-triol + NAD(P)+ + H2O + CO2

2 entries

3,4-Dihydroxybenzoate + NADH + O2

1,2,4-Trihydroxybenzene + NAD+ + CO2

Candida parapsilosis

-

-

7428

-

7428

?

3,4-Dihydroxybenzoate + NADPH + O2

1,2,4-Trihydroxybenzene + NADP+ + CO2

Candida parapsilosis

-

-

7428

-

7428

?

3,5-Difluoro-4-hydroxybenzoate + NADH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

3,5-Difluoro-4-hydroxybenzoate + NADPH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

3-Amino-4-hydroxybenzoate + NADH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

3-Amino-4-hydroxybenzoate + NADPH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

3-Chloro-4-hydroxybenzoate + NADH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

3-Chloro-4-hydroxybenzoate + NADPH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

3-Fluoro-4-hydroxybenzoate + NADH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

3-Fluoro-4-hydroxybenzoate + NADPH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

4-Hydroxy-3-methoxybenzoate + NADH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

4-Hydroxy-3-methoxybenzoate + NADPH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

4-hydroxy-3-nitrobenzoate + NAD(P)H + O2

2-nitrobenzene-1,4-diol + NAD(P)+ + CO2

2 entries

4-Hydroxy-3-nitrobenzoate + NADH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

4-Hydroxy-3-nitrobenzoate + NADPH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

4-hydroxybenzoate + NAD(P)H + 2 H+ + O2

hydroquinone + NAD(P)+ + H2O + CO2

Yarrowia lipolytica

-

-

764953

-

-

?

4-hydroxybenzoate + NAD(P)H + H+ + O2

hydroquinone + NAD(P)+ + H2O + CO2

2 entries

4-Hydroxybenzoate + NAD(P)H + O2

?

3 entries

4-hydroxybenzoate + NADH + 2 H+ + O2

hydroquinone + NAD+ + H2O + CO2

4 entries

4-Hydroxybenzoate + NADH + O2

1,4-Dihydroxybenzene + NAD+ + CO2

4 entries

4-hydroxybenzoate + NADH + O2

hydroquinone + NAD+ + CO2

2 entries

4-Hydroxybenzoate + NADPH + O2

1,4-Dihydroxybenzene + NADP+ + CO2

4 entries

4-hydroxybenzoate + NADPH + O2

hydroquinone + NADP+ + CO2

2 entries

protocatechuate + NAD(P)H + H+ + O2

?

2 entries

protocatechuate + NADH + 2 H+ + O2

hydroquinone + NAD+ + H2O + CO2

2 entries

tetrafluoro-4-hydroxybenzoate + NAD(P)H + O2

2,3,5,6-tetrafluorohydroquinone + NAD(P)+ + CO2

Candida parapsilosis

-

-

657498

-

-

?

vanillate + NADH + 2 H+ + O2

3-methoxy-1,4-dihydroxybenzene + NAD+ + H2O + CO2

2 entries

additional information

?

-

6 entries

2,4-dihydroxybenzoate + NAD(P)H + H+ + O2

benzene-1,2,4-triol + NAD(P)+ + H2O + CO2

Candida parapsilosis

-

-

728255

-

-

?

2,4-dihydroxybenzoate + NAD(P)H + H+ + O2

benzene-1,2,4-triol + NAD(P)+ + H2O + CO2

Candida parapsilosis CDU1

-

-

728255

-

-

?

2,4-Dihydroxybenzoate + NADH + O2

1,2,4-Trihydroxybenzene + NAD+ + CO2

Candida parapsilosis

-

-

7428

-

7428

?

2,4-Dihydroxybenzoate + NADH + O2

1,2,4-Trihydroxybenzene + NAD+ + CO2

Candida parapsilosis CBS604

-

-

7428

-

7428

?

2-chloro-4-hydroxybenzoate + NADH + O2

?

Candida parapsilosis

-

-

7428

-

-

?

2-chloro-4-hydroxybenzoate + NADH + O2

?

Candida parapsilosis CBS604

-

-

7428

-

-

?

2-fluoro-4-hydroxybenzoate + NADH + O2

2-fluoro-1,4-dihydroxybenzene + NAD+ + CO2

Candida parapsilosis

-

-

7428

-

7428

?

2-fluoro-4-hydroxybenzoate + NADH + O2

2-fluoro-1,4-dihydroxybenzene + NAD+ + CO2

Candida parapsilosis CBS604

-

-

7428

-

7428

?

3,4-dihydroxybenzoate + NAD(P)H + H+ + O2

benzene-1,2,4-triol + NAD(P)+ + H2O + CO2

Candida parapsilosis

-

-

728255

-

-

?

3,4-dihydroxybenzoate + NAD(P)H + H+ + O2

benzene-1,2,4-triol + NAD(P)+ + H2O + CO2

Candida parapsilosis CDU1

-

-

728255

-

-

?

4-hydroxy-3-nitrobenzoate + NAD(P)H + O2

2-nitrobenzene-1,4-diol + NAD(P)+ + CO2

Candida parapsilosis

-

-

657498

-

-

?

4-hydroxy-3-nitrobenzoate + NAD(P)H + O2

2-nitrobenzene-1,4-diol + NAD(P)+ + CO2

Candida parapsilosis CBS604

-

-

657498

-

-

?

4-hydroxybenzoate + NAD(P)H + H+ + O2

hydroquinone + NAD(P)+ + H2O + CO2

Candida parapsilosis

-

-

728255

-

-

?

4-hydroxybenzoate + NAD(P)H + H+ + O2

hydroquinone + NAD(P)+ + H2O + CO2

Candida parapsilosis CDU1

-

-

728255

-

-

?

4-Hydroxybenzoate + NAD(P)H + O2

?

Candida parapsilosis

-

initial reaction of the catabolism of 4-hydroxybenzoate

7427

-

-

?

4-Hydroxybenzoate + NAD(P)H + O2

?

Candida parapsilosis

-

the enzyme is induced when the yeast is grown on either 4-hydroxybenzoate, 2,3-dihydroxybenzoate, or 3,4-dihydroxybenzoate as the sole carbon source

7428

-

-

?

4-Hydroxybenzoate + NAD(P)H + O2

?

Candida parapsilosis CBS604

-

initial reaction of the catabolism of 4-hydroxybenzoate

7427

-

-

?

4-hydroxybenzoate + NADH + 2 H+ + O2

hydroquinone + NAD+ + H2O + CO2

Candida parapsilosis

G8B709

-

745691

-

-

?

4-hydroxybenzoate + NADH + 2 H+ + O2

hydroquinone + NAD+ + H2O + CO2

Candida parapsilosis

-

-

764036

-

-

?

4-hydroxybenzoate + NADH + 2 H+ + O2

hydroquinone + NAD+ + H2O + CO2

Candida parapsilosis CBS604

G8B709

-

745691

-

-

?

4-hydroxybenzoate + NADH + 2 H+ + O2

hydroquinone + NAD+ + H2O + CO2

Candida parapsilosis CBS604

-

-

764036

-

-

?

4-Hydroxybenzoate + NADH + O2

1,4-Dihydroxybenzene + NAD+ + CO2

Candida parapsilosis

-

-

7427

-

7427

?

4-Hydroxybenzoate + NADH + O2

1,4-Dihydroxybenzene + NAD+ + CO2

Candida parapsilosis

-

NADH is the preferred electron donor

7428

-

7428

?

4-Hydroxybenzoate + NADH + O2

1,4-Dihydroxybenzene + NAD+ + CO2

Candida parapsilosis CBS604

-

-

7427

-

7427

?

4-Hydroxybenzoate + NADH + O2

1,4-Dihydroxybenzene + NAD+ + CO2

Candida parapsilosis CBS604

-

NADH is the preferred electron donor

7428

-

7428

?

4-hydroxybenzoate + NADH + O2

hydroquinone + NAD+ + CO2

Candida parapsilosis

-

the enzyme prefers NADH over NADPH as electron donor

657498

-

-

?

4-hydroxybenzoate + NADH + O2

hydroquinone + NAD+ + CO2

Candida parapsilosis CBS604

-

the enzyme prefers NADH over NADPH as electron donor

657498

-

-

?

4-Hydroxybenzoate + NADPH + O2

1,4-Dihydroxybenzene + NADP+ + CO2

Candida parapsilosis

-

-

7427

-

7427

?

4-Hydroxybenzoate + NADPH + O2

1,4-Dihydroxybenzene + NADP+ + CO2

Candida parapsilosis

-

NADH is the preferred electron donor

7428

-

7428

?

4-Hydroxybenzoate + NADPH + O2

1,4-Dihydroxybenzene + NADP+ + CO2

Candida parapsilosis CBS604

-

-

7427

-

7427

?

4-Hydroxybenzoate + NADPH + O2

1,4-Dihydroxybenzene + NADP+ + CO2

Candida parapsilosis CBS604

-

NADH is the preferred electron donor

7428

-

7428

?

4-hydroxybenzoate + NADPH + O2

hydroquinone + NADP+ + CO2

Candida parapsilosis

-

the enzyme prefers NADH over NADPH as electron donor

657498

-

-

?

4-hydroxybenzoate + NADPH + O2

hydroquinone + NADP+ + CO2

Candida parapsilosis CBS604

-

the enzyme prefers NADH over NADPH as electron donor

657498

-

-

?

protocatechuate + NAD(P)H + H+ + O2

?

Candida parapsilosis

-

-

728255

-

-

?

protocatechuate + NAD(P)H + H+ + O2

?

Candida parapsilosis CDU1

-

-

728255

-

-

?

protocatechuate + NADH + 2 H+ + O2

hydroquinone + NAD+ + H2O + CO2

Candida parapsilosis

-

-

764036

-

-

?

protocatechuate + NADH + 2 H+ + O2

hydroquinone + NAD+ + H2O + CO2

Candida parapsilosis CBS604

-

-

764036

-

-

?

vanillate + NADH + 2 H+ + O2

3-methoxy-1,4-dihydroxybenzene + NAD+ + H2O + CO2

Candida parapsilosis

-

-

764036

-

-

?

vanillate + NADH + 2 H+ + O2

3-methoxy-1,4-dihydroxybenzene + NAD+ + H2O + CO2

Candida parapsilosis CBS604

-

-

764036

-

-

?

additional information

?

-

Candida parapsilosis

-

the enzyme is induced when yeast is grown on either 4-hydroxybenzoate, 2,4-dihydroxybenzoate or 3,4-dihydroxybenzoate as the sole carbon source

657498

-

-

?

additional information

?

-

Candida parapsilosis

-

the phenolate form of the substrates is important for catalysis and the substrate hydroxylation involvesthe electrophilic attack of the putative flavin hydroperoxide at the C1-atom of the aromatic ring

657498

-

-

?

additional information

?

-

Candida parapsilosis

-

4HB1H catalyzes an oxidative decarboxylation reaction. 4HB1H is active with a wide range of 4-hydroxybenzoate (4-HB) derivatives. 4HB1H can also be induced by protocatechuate (PCA) and convert this compound directly into the central intermediate HHQ

764036

-

-

-

additional information

?

-

Candida parapsilosis CBS604

-

4HB1H catalyzes an oxidative decarboxylation reaction. 4HB1H is active with a wide range of 4-hydroxybenzoate (4-HB) derivatives. 4HB1H can also be induced by protocatechuate (PCA) and convert this compound directly into the central intermediate HHQ

764036

-

-

-

additional information

?

-

Candida parapsilosis CBS604

-

the enzyme is induced when yeast is grown on either 4-hydroxybenzoate, 2,4-dihydroxybenzoate or 3,4-dihydroxybenzoate as the sole carbon source

657498

-

-

?

additional information

?

-

Candida parapsilosis CBS604

-

the phenolate form of the substrates is important for catalysis and the substrate hydroxylation involvesthe electrophilic attack of the putative flavin hydroperoxide at the C1-atom of the aromatic ring

657498

-

-

?