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1.13.11.8: protocatechuate 4,5-dioxygenase

This is an abbreviated version!
For detailed information about protocatechuate 4,5-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.8

Reaction

3,4-dihydroxybenzoate
+
O2
=
4-carboxy-2-hydroxymuconate semialdehyde

Synonyms

LigAB, PCA 4,5-dioxygenase, PcaA, PCD 4,5, PCD4,5, PmdAB, protocatechuate 4,5-oxygenase, protocatechuic 4,5-dioxygenase, protocatechuic 4,5-oxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.8 protocatechuate 4,5-dioxygenase

Cloned

Cloned on EC 1.13.11.8 - protocatechuate 4,5-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene organization of the PCA45 pathway genes in strain E6, overview. Identification of the pmd promoter region, the transcription start site of the pmd operon is a thymine 167 nt upstream of the pmdU start codon, overview
-
genetic organization Comamonas-type with a single operon
-
genetic organization Sphingobium-type with several transcriptional units
-
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha