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1.13.11.8: protocatechuate 4,5-dioxygenase

This is an abbreviated version!
For detailed information about protocatechuate 4,5-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.8

Reaction

3,4-dihydroxybenzoate
+
O2
=
4-carboxy-2-hydroxymuconate semialdehyde

Synonyms

LigAB, PCA 4,5-dioxygenase, PcaA, PCD 4,5, PCD4,5, PmdAB, protocatechuate 4,5-oxygenase, protocatechuic 4,5-dioxygenase, protocatechuic 4,5-oxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.8 protocatechuate 4,5-dioxygenase

Purification

Purification on EC 1.13.11.8 - protocatechuate 4,5-dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Q-Sepharose
-
heat treatment, DEAE-Sepharose, superdex 200, Resource Q
recombinant His6-tagged wild-type and mutant enzymes anaerobically from Escherichia coli strain DH5alpha by nickel affinity chromatography, tag cleavage anaerobically by thrombin cleavage