EC Number   |
Protein Variants |
Reference |
|---|
   5.4.99.9 | D322A |
site-directed mutagenesis, the mutant shows altered kinetics compared to the wild-type with substrate UDP-alpha-D-galactofuranose |
747540 |
| 5.4.99.9 | D351A |
mutation of conserved residue of putative active site cleft. 4% of wild-type activity |
678290 |
| 5.4.99.9 | E301A |
mutation of conserved residue of putative active site cleft. 21% of wild-type activity |
678290 |
| 5.4.99.9 | F66A |
site-directed mutagenesis, the mutant enzyme shows a high decrease in kcat/KM value compared to wild-type, but the mutation does not significantly affect the kinetics of enzyme activation by NADPH. Crystal structure determination of the enzyme ligand-free or in complex with UDP or UDP and substrate UDP-alpha-D-galactopyranose |
747079 |
| 5.4.99.9 | F66A |
site-directed mutagenesis, the residue is located at the end of AfUgmA loop III and may control loop III flipping and consequently opening of the mobile loops depending on the redox state of the cofactor. The colony morphology, cell wall composition, hyphal surface adhesion and response to antifungal drugs of the mutant are altered compared to wild-type |
749145 |
| 5.4.99.9 | H63A |
site-directed mutagenesis, inactive mutant |
747117 |
| 5.4.99.9 | H63N |
site-directed mutagenesis, the residue is part of the flexible loop (loop III) above the si-face of the isoalloxazine ring. The colony morphology, cell wall composition, hyphal surface adhesion and response to antifungal drugs of the mutant are altered compared to wild-type |
749145 |
| 5.4.99.9 | H63N |
site-directed mutagenesis, the structure of enzyme mutant AfUGMH63A complexed with the substrate UDP-Galp shows the presence of a C1-galactose-N5-FAD adduct (PDB ID 5HHF) |
746922 |
| 5.4.99.9 | H68A |
site-directed mutagenesis, the mutant shows highly reduced catalytic efficiency compared to wild-type |
747079 |
| 5.4.99.9 | more |
compared to a wild type morphology strain, the UGM-deficient ugmA? strain has aberrant hyphal morphology, producing wide, uneven, highly-branched hyphae, with thick, relatively electron-dense walls |
692458 |
