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Literature summary for 5.4.99.9 extracted from

  • Chad, J.M.; Sarathy, K.P.; Gruber, T.D.; Addala, E.; Kiessling, L.L.; Sanders, D.A.
    Site-directed mutagenesis of UDP-galactopyranose mutase reveals a critical role for the active-site, conserved arginine residues (2007), Biochemistry, 46, 6723-6732.
    View publication on PubMed
Search for more literature for 5.4.99.9

Cloned(Commentary)

Cloned (Comment) Organism
expression with His-tag in Escherichai coli Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
D351A mutation of conserved residue of putative active site cleft. 4% of wild-type activity Klebsiella pneumoniae
E301A mutation of conserved residue of putative active site cleft. 21% of wild-type activity Klebsiella pneumoniae
R174A no catalytic activity Klebsiella pneumoniae
R280A mutation of conserved residue of putative active site cleft. No catalytic activity Klebsiella pneumoniae
W160A mutation of conserved residue on edge putative active site cleft, defective in binding of substrate Klebsiella pneumoniae
Y155F 7% of wild-type activity Klebsiella pneumoniae
Y185F 11% of wild-type activity Klebsiella pneumoniae
Y314F 5% of wild-type activity Klebsiella pneumoniae
Y349F 6% of wild-type activity Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 UDP-D-galactopyranose wild-type, pH 8.0 Klebsiella pneumoniae
0.205
-
 UDP-D-galactopyranose mutant E301A, pH 8.0 Klebsiella pneumoniae
0.386
-
 UDP-D-galactopyranose mutant Y185F, pH 8.0 Klebsiella pneumoniae
0.619
-
 UDP-D-galactopyranose mutant Y155F, pH 8.0 Klebsiella pneumoniae
0.739
-
 UDP-D-galactopyranose mutant Y349F, pH 8.0 Klebsiella pneumoniae
0.819
-
 UDP-D-galactopyranose mutant Y314F, pH 8.0 Klebsiella pneumoniae
1.002
-
 UDP-D-galactopyranose mutant D351A, pH 8.0 Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae Q48485
-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose mobile loop of enzyme must move in order for enzyme to bind UDP-galactose substrate Klebsiella pneumoniae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-galactopyranose
-
 Klebsiella pneumoniae UDP-D-galactofuranose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 UDP-D-galactopyranose mutant D351A, pH 8.0 Klebsiella pneumoniae
0.3
-
 UDP-D-galactopyranose mutant E301A, pH 8.0 Klebsiella pneumoniae
1.3
-
 UDP-D-galactopyranose mutant Y185F, pH 8.0 Klebsiella pneumoniae
1.7
-
 UDP-D-galactopyranose mutant Y349F, pH 8.0 Klebsiella pneumoniae
3.6
-
 UDP-D-galactopyranose mutant Y155F, pH 8.0 Klebsiella pneumoniae
5.2
-
 UDP-D-galactopyranose mutant Y314F, pH 8.0 Klebsiella pneumoniae
5.5
-
 UDP-D-galactopyranose wild-type, pH 8.0 Klebsiella pneumoniae