EC Number   |
Protein Variants |
Reference |
|---|
   4.1.2.9 | H260Y |
47.8% increase in vmax compared to wild-type |
762660 |
| 4.1.2.9 | more |
since Lactobacillus plantarum has two phosphoketolase genes, xpk1 and xpk2, a delta xpk2 mutant is structured from Lactobacillus plantarum delta ldhL1-xpk1::tkt/pCU-PXylAB. Disruption of xpk2 causes a slight decrease in cell growth and fermentation rates, complete abolition of enzymes that have phosphoketolase activity, combined with introduction of enzymes that function as a bridge to the pentose phosphate pathway, enables homo-lactic acid production by pentose sugar fermentation |
-, 701818 |
| 4.1.2.9 | more |
the metabolic pathway in L-lactate dehydrogenase gene-deficient Lactobacillus plantarum NCIMB 8826 is engineered, redirection of the phosphoketolase pathway to the pentose phosphate pathway in Lactobacillus plantarum delta ldhL1 by substituting an endogenous phosphoketolase gene (xpk1) with the heterologous transketolase gene (tkt) from Lactococcus lactis IL 1403 is achieved, thereby shifts heterolactic acid fermentation to homolactic acid fermentation |
-, 701801 |
| 4.1.2.9 | synthesis |
highest polyhydroxybutyrate production by Saccharomyces cerevisiae after 100 h is achieved with a strain harboring the phosphoketolase pathway, i.e xylulose-5-phosphate phosphoketolase EC 4.1.2.9 and acetate kinase EC 2.7.2.1 from Emericella nidulans and an acetyl-CoA synthetase variant L641P from Salmonella enterica on a single episomal plasmid to supply acetyl-CoA without the need of increased NADPH production by gapN integration |
727211 |
| 4.1.2.9 | T2A/I6T |
59.1% increase in vmax compared to wild-type |
762660 |
| 4.1.2.9 | T2A/I6T/H260Y |
73.3% increase in vmax compared to wild-type |
762660 |
