Literature summary for 4.1.2.9 extracted from

Dele-Osibanjo, T.; Li, Q.; Zhang, X.; Guo, X.; Feng, J.; Liu, J.; Sun, X.; Wang, X.; Zhou, W.; Zheng, P.; Sun, J.; Ma, Y.
Growth-coupled evolution of phosphoketolase to improve L-glutamate production by Corynebacterium glutamicum (2019), Appl. Microbiol. Biotechnol., 103, 8413-8425 .

Search for more literature for 4.1.2.9

Application

Application	Comment	Organism
synthesis	expression of mutant T2A/I6T/H260 in Corynebacterium glutamicum Z188 results in 16.67% and 18.19% improvement in L-glutamate titer and yield, respectively, compared with the wildtype	Bifidobacterium adolescentis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Corynebacterium glutamicum	Bifidobacterium adolescentis

Protein Variants

Protein Variants	Comment	Organism
H260Y	47.8% increase in vmax compared to wild-type	Bifidobacterium adolescentis
T2A/I6T	59.1% increase in vmax compared to wild-type	Bifidobacterium adolescentis
T2A/I6T/H260Y	73.3% increase in vmax compared to wild-type	Bifidobacterium adolescentis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
9.73	-	D-fructose 6-phosphate	mutant T2A/I6T, pH 6.5, 30°C	Bifidobacterium adolescentis
9.84	-	D-fructose 6-phosphate	mutant T2A/I6T/H260Y, pH 6.5, 30°C	Bifidobacterium adolescentis
9.86	-	D-fructose 6-phosphate	mutant H260Y, pH 6.5, 30°C	Bifidobacterium adolescentis
9.96	-	D-fructose 6-phosphate	wild-type, pH 6.5, 30°C	Bifidobacterium adolescentis

Organism

Organism	UniProt	Comment	Textmining
Bifidobacterium adolescentis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Fructose 6-phosphate + phosphate	-	Bifidobacterium adolescentis	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-xylulose 5-phosphate + phosphate	-	Bifidobacterium adolescentis	acetyl phosphate + D-glyceraldehyde 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
FXPK	-	Bifidobacterium adolescentis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.73	-	D-fructose 6-phosphate	wild-type, pH 6.5, 30°C	Bifidobacterium adolescentis
2.71	-	D-fructose 6-phosphate	mutant H260Y, pH 6.5, 30°C	Bifidobacterium adolescentis
2.88	-	D-fructose 6-phosphate	mutant T2A/I6T, pH 6.5, 30°C	Bifidobacterium adolescentis
3.07	-	D-fructose 6-phosphate	mutant T2A/I6T/H260Y, pH 6.5, 30°C	Bifidobacterium adolescentis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.17	-	D-fructose 6-phosphate	wild-type, pH 6.5, 30°C	Bifidobacterium adolescentis
0.28	-	D-fructose 6-phosphate	mutant H260Y, pH 6.5, 30°C	Bifidobacterium adolescentis
0.3	-	D-fructose 6-phosphate	mutant T2A/I6T, pH 6.5, 30°C	Bifidobacterium adolescentis
0.32	-	D-fructose 6-phosphate	mutant T2A/I6T/H260Y, pH 6.5, 30°C	Bifidobacterium adolescentis

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Release 2023.1 (January 2023)