EC Number   |
Protein Variants |
Reference  |
|---|
    4.1.2.43 | more |
in order to improve the rate of vanillin degradation by Burkholderia cepacia TM1, the hps and phi genes from Methylomonas aminofaciens 77a are heterologously expressed in strain TM1, the transformant strain constitutively produces active 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase enzymes and the degradation of vanillic acid and the growth yield are significantly improved |
-, 701850 |
| 4.1.2.43 | more |
the hps and phi genes from the methylotrophic bacterium Mycobacterium gastri MB19 are introduced into tobacco. Both genes are expressed under the control of the tomato rbcS-3C promoter, and the gene products are targeted to the chloroplasts by artificially added transit peptide sequence, the expression of both genes in plants enhances the tolerance of the transgenic plant to formaldehyde and capability of eliminating environmental formaldehyde |
-, 701850 |
| 4.1.2.43 | more |
the solvent-tolerant bacterium, Pseudomonas putida S12, is engineered to efficiently utilize methanol and formaldehyde as auxiliary substrates by introducing the hps and phi genes from the thermotolerant methylotrophic bacterium Bacillus brevis, by chemostat culture experiments using glucose and formaldehyde, the hps and phi-expressing strain shows both significantly improved cell mass and growth at higher formaldehyde concentrations than the control strain |
-, 701850 |
| 4.1.2.43 | H134A |
the mutation abolishes HPS activity |
-, 716871 |
| 4.1.2.43 | H134D |
the mutation abolishes HPS activity |
-, 716871 |
| 4.1.2.43 | H134K |
the mutation abolishes HPS activity |
-, 716871 |
| 4.1.2.43 | H134N |
the mutation abolishes HPS activity |
-, 716871 |
| 4.1.2.43 | H134S |
the mutation abolishes HPS activity |
716871 |
| 4.1.2.43 | K61A |
the mutant exhibits 50-90% of the activity of the wild type enzyme |
-, 716871 |
| 4.1.2.43 | K61D |
the mutant exhibits 50-90% of the activity of the wild type enzyme |
716871 |
