EC Number   |
Protein Variants |
Reference |
|---|
   3.5.1.124 | C106S |
site-directed mutagenesis, inactive mutant |
740735 |
| 3.5.1.124 | C188A |
immeasurable glyoxalase activity |
-, 755138 |
| 3.5.1.124 | C46S |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
740735 |
| 3.5.1.124 | C53S |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
740735 |
| 3.5.1.124 | E79A |
immeasurable glyoxalase activity |
-, 755138 |
| 3.5.1.124 | H158A |
reduced amidopeptidase activity, glyoxalase activity is 53% compared to wild-type enzyme |
-, 755138 |
| 3.5.1.124 | H189A |
immeasurable glyoxalase activity |
-, 755138 |
| 3.5.1.124 | H76A |
immeasurable glyoxalase activity |
-, 755138 |
| 3.5.1.124 | more |
construction of DJ-1 knockdown in Drosophila cells in culture, and DJ-1beta knock-out flies. DJ-1beta knockdown does not affect the response of S2 cells to methylglyoxal challenge |
740762 |
| 3.5.1.124 | more |
construction of yhbO/yajL and yhbO/yajL/hchA mutants displaying high amounts of glycated proteins |
739997 |
