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EC Number
Protein Variants
Commentary
Reference
3.4.24.55
D79A
no effect on function
667533
3.4.24.55
E91A
no proteolytic activity
667533
3.4.24.55
F198A
enzyme remains functional
667533
3.4.24.55
H193A
enzyme remains functional
667533
3.4.24.55
K197A
enzyme remains functional
667533
3.4.24.55
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heterologous expression of enzyme in yeast, enzyme functionally substitutes for yeast Ax11p and Ste23p in pheromone production. Independent heterologous expression of N-terminal domain with amino acids 1-515 or C-terminal domain with amino acids 516-962 does not promote yeast mating. Co-expression of both domains restores yeast mating
667533
3.4.24.55
N119A
promotion of yeast mating factor in heterologous expression is disabled
667533
3.4.24.55
N317A/R318A/S319A
enzyme remains functional
667533
3.4.24.55
R792A
no proteolytic activity
667533
3.4.24.55
R792A/T793A/E794A/E795A
no proteolytic activity
667533
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