EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.76 | F180A/F181A |
mutant is still able to display membrane PR3 after degranulation, with similar fluorescence intensity as wild-type |
717959 |
| 3.4.21.76 | F180A/F181A/L228A/F229A |
contrary to wild-type, membrane anchorage is abrogated in mutant. Mutant is still able to cleave the synthetic substrate Boc-Ala-Pro-Val in cell lysates, but fails to cleave extracellular fibronectin, is not externalized after apoptosis and does not impair macrophage phagocytosis of apoptotic cells, does not promote myeloid cell proliferation and fails to cleave p21/waf1 |
717959 |
| 3.4.21.76 | F180A/L228A/F229A |
mutant is still able to display membrane PR3 after degranulation, with similar fluorescence intensity as wild-type |
717959 |
| 3.4.21.76 | H4A |
site-directed mutagenesis |
754130 |
| 3.4.21.76 | L228A |
mutant is still able to display membrane PR3 after degranulation, with similar fluorescence intensity as wild-type |
717959 |
| 3.4.21.76 | L228A/F229A |
mutant is still able to display membrane PR3 after degranulation, with similar fluorescence intensity as wild-type |
717959 |
| 3.4.21.76 | more |
catalytically inactive Ser195-substituted PR3 variant, eliminates quality check of the antigen preperation. Recombinant PR3 variants which only carry a single antigenic region on its surface may be used to neutralize or eliminate circulating anti-neutrophil cytoplasmic antibodies (ANCA) in patients without triggering immune complex-mediated type III reactions |
686188 |
| 3.4.21.76 | N102Q |
glycosylation deficient recombinant PR3 mutant, hydrolytic activity like recombinant PR3-wild-type. N-terminal processing is delayed |
687439 |
| 3.4.21.76 | N102Q/N147Q |
glycosylation deficient recombinant PR3 mutant, reduction in hydrolytic activity in relation to recombinant PR3-wild-type. Reduction in hydrolytic activity is more pronounced than the reduction induced by deglycosylation of purified neutrophil PR3. N-terminal processing is delayed |
687439 |
| 3.4.21.76 | N147Q |
glycosylation deficient recombinant PR3 mutant, is processed like recombinant PR3-wild-type |
687439 |
