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Literature summary for 3.4.21.76 extracted from
- Functional significance of Asn-linked glycosylation of proteinase 3 for enzymatic activity, processing, targeting, and recognition by anti-neutrophil cytoplasmic antibodies (2007), J. Biochem., 141, 101-112.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | glycosylation at Asn-147, but not at Asn-102, is critical for optimal hydrolytic activity of PR3. Efficient amino-terminal proteolytic processing of recombinant PR3 is dependent on glycosylation at Asn-102 | Homo sapiens |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | in patients with Wegeners granulomatosis, epitope recognition by anti-neutrophil cytoplasmic antibodies (ANCA) is affected by the glycosylation status of PR3 | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant PR3 wild-type and recombinant PR3 glycosylation variants expressed in HMC-1 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N102Q | glycosylation deficient recombinant PR3 mutant, hydrolytic activity like recombinant PR3-wild-type. N-terminal processing is delayed | Homo sapiens |
| N102Q/N147Q | glycosylation deficient recombinant PR3 mutant, reduction in hydrolytic activity in relation to recombinant PR3-wild-type. Reduction in hydrolytic activity is more pronounced than the reduction induced by deglycosylation of purified neutrophil PR3. N-terminal processing is delayed | Homo sapiens |
| N147Q | glycosylation deficient recombinant PR3 mutant, is processed like recombinant PR3-wild-type | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | hydrolysis of N-methoxysuccinyl-Ala-Ala-Pro-Val-pNA by neutrophil PR3 is reduced by 40% as a result of deglycosylation | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
recombinant mutant N102Q/N147Q, immunoprecipitation | Homo sapiens |
| 32000 | - |
recombinant mutant N102Q and N147Q, immunoprecipitation | Homo sapiens |
| 34000 | - |
recombinant PR3 wild-type, immunoprecipitation | Homo sapiens |
| 36000 | - |
recombinant PR3 wild-type, immunoprecipitation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| neutrophil | - |
Homo sapiens | - |
| neutrophil | targeting to granules is not dependent on glycosylation, but unglycosylated recombinant PR3 gets secreted preferentially into media supernatants | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-methoxysuccinyl-Ala-Ala-Pro-Val-pNA + H2O | - |
Homo sapiens | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PR3 | - |
Homo sapiens |
| proteinase 3 | - |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
glycosylation at Asn-147, but not at Asn-102, is critical for thermal stability | Homo sapiens |
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