3.4.17.14 | more |
the enzyme mutant K23 of Brucella abortus fails to replicate in mouse macrophages and HeLa cells, and shows less virulence than the wild type in mice, Under nitric oxide (NO) stress, the growth of the DAP mutant in vitro decreases, and it also has less capability to reduce NO than the wild type, intracellular replication of the DAP mutant is partially restored by pretreatment of macrophages with the NO synthase inhibitor 1-phenyl-imidazole and the level of expression of the NO reductase gene norB in the DAP mutant is lower than that in the wild type |
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