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Literature summary for 3.4.17.14 extracted from
- The DD-carboxypeptidase activity encoded by pbp4B is not essential for the cell growth of Escherichia coli (2006), Arch. Microbiol., 185, 23-27.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pbp4B, DNA and amino acid sequence determination and analysis, the pbp4B gene is located at the zipA-dapA region, subcloning in strain DH5alpha, expression of N-terminally His-tagged enzyme in strain BL21(DE3) membranes | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of gene pbp4B deletion mutants, the enzyme is not not essential for cell division, the strain lacking all known D-alanine carboxypeptidases is viable, overview | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50978 | - |
x * 50978, sequence calculation | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | the DD-carboxypeptidase activity encoded by pbp4B is not essential for the cell growth of Escherichia coli | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene pbp4B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from strain BL21(DE3) membranes by solubilization with 1% Triton X-100 and nickel affinity chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the DD-carboxypeptidase activity encoded by pbp4B is not essential for the cell growth of Escherichia coli | Escherichia coli | ? | - |
? | |
| additional information | PBP4B shows penicillin-binding capacity and a low DD-carboxypeptidase activity | Escherichia coli | ? | - |
? | |
| Nalpha,Nepsilon-diacetyl-Lys-D-Ala-D-Ala + H2O | - |
Escherichia coli | Nalpha,Nepsilon-diacetyl-Lys-D-Ala + D-Ala | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50978, sequence calculation | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DD-Carboxypeptidase | - |
Escherichia coli |
| More | the enzyme belongs to the serine protease family | Escherichia coli |
| PBP4B | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | sequence calculation | - |
8.03 |
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Release 2023.1 (January 2023)
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