EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.99 | D147A |
site-directed mutagenesis, structure comparison with the wild-type enzyme |
-, 707433 |
| 3.2.1.99 | D147N |
inactive mutant enzyme |
749521 |
| 3.2.1.99 | D171A |
the mutant of isoform Arb43B displays no measurable activity |
714964 |
| 3.2.1.99 | D27A |
inactive mutant enzyme |
749521 |
| 3.2.1.99 | D38A |
the mutant of isoform Arb43B displays no measurable activity |
714964 |
| 3.2.1.99 | DELTAV2-W17 |
deletion of the N-terminal amino acids causes thermal instability |
-, 665426 |
| 3.2.1.99 | E201A |
site-directed mutagenesis, structure comparison with the wild-type enzyme |
-, 707433 |
| 3.2.1.99 | E224A |
the mutant of isoform Arb43B displays no measurable activity |
714964 |
| 3.2.1.99 | H218D |
the mutant shows higher catalytic efficiency, kcat is improved about 45% versus that of the wild type enzyme |
731976 |
| 3.2.1.99 | H218E |
the mutant is active, but it does not change the enzymatic property obviously under acidic condition compared with the wild type enzyme |
731976 |
