Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Paenibacillus polymyxa |
Protein Variants | Comment | Organism |
---|---|---|
H218D | the mutant shows higher catalytic efficiency, kcat is improved about 45% versus that of the wild type enzyme | Paenibacillus polymyxa |
H218E | the mutant is active, but it does not change the enzymatic property obviously under acidic condition compared with the wild type enzyme | Paenibacillus polymyxa |
H218K | inactive | Paenibacillus polymyxa |
H218R | inactive | Paenibacillus polymyxa |
H297D | inactive | Paenibacillus polymyxa |
H297E | inactive | Paenibacillus polymyxa |
H297K | inactive | Paenibacillus polymyxa |
H297R | inactive | Paenibacillus polymyxa |
H48D | inactive | Paenibacillus polymyxa |
H48E | inactive | Paenibacillus polymyxa |
H48K | inactive | Paenibacillus polymyxa |
H48R | inactive | Paenibacillus polymyxa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1177 | - |
linear 1,5-alpha-L-arabinan | at 40°C and pH 6.0 | Paenibacillus polymyxa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35477 | - |
x * 35477, calculated from amino acid sequence | Paenibacillus polymyxa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus polymyxa | V5LER1 | - |
- |
Paenibacillus polymyxa Z6 | V5LER1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography | Paenibacillus polymyxa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
73.4 | - |
with linear linear 1,5-alpha-L-arabinan as substrate, at 40°C, pH 6.0 | Paenibacillus polymyxa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linear 1,5-alpha-L-arabinan + H2O | - |
Paenibacillus polymyxa | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
linear 1,5-alpha-L-arabinan + H2O | - |
Paenibacillus polymyxa Z6 | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
additional information | no activity with 4-nitrophenyl-alpha-L-arabinofuranoside, beechwood xylan, wheat flour arabinoxylan, larch arabinogalactan, gum arabic, and soluble starch | Paenibacillus polymyxa | ? | - |
? | |
additional information | no activity with 4-nitrophenyl-alpha-L-arabinofuranoside, beechwood xylan, wheat flour arabinoxylan, larch arabinogalactan, gum arabic, and soluble starch | Paenibacillus polymyxa Z6 | ? | - |
? | |
red debranched arabinan + H2O | - |
Paenibacillus polymyxa | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
red debranched arabinan + H2O | - |
Paenibacillus polymyxa Z6 | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
sugar beet arabinan + H2O | - |
Paenibacillus polymyxa | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
sugar beet arabinan + H2O | - |
Paenibacillus polymyxa Z6 | oligo-alpha-(1->5)-L-arabinoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35477, calculated from amino acid sequence | Paenibacillus polymyxa |
Synonyms | Comment | Organism |
---|---|---|
AbnZ1 | - |
Paenibacillus polymyxa |
endo-1,5-alpha-L-arabinase | - |
Paenibacillus polymyxa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Paenibacillus polymyxa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
the specific activity of the enzyme remains 54.1% of maximum at 5°C | Paenibacillus polymyxa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
68.7 | - |
linear 1,5-alpha-L-arabinan | wild type enzyme, at 40°C and pH 6.0 | Paenibacillus polymyxa | |
99.7 | - |
linear 1,5-alpha-L-arabinan | mutant enzyme H218D, at 40°C and pH 6.0 | Paenibacillus polymyxa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Paenibacillus polymyxa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | the enzyme achieves about 20.4 and 83.6% of the maximal activity at pH 5.0 and 7.0, respectively | Paenibacillus polymyxa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | the enzyme is stable at pH 7.0 compared with 55.5 and 5.78% of maximal activity remaining after 30 min of incubation at pH 6.0 and 5.0 respectively | Paenibacillus polymyxa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Paenibacillus polymyxa | calculated from amino acid sequence | - |
9.2 |