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Results 1 - 10 of 39 > >>
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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.79D131A 2–3% of wild-type activity 711199
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.79D153A poorly expressed. D153A is stable, but its expression is somehow toxic to Escherichia coli. 312% of wild-type G(-1) addition activity. G(-) i.e. the extra nucleotide posttranscriptionally added in position (-1) of tRNA 711199
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.79D47A 54.7% of wild-type G(-1) addition activity. G(-) i.e. the extra nucleotide posttranscriptionally added in position (-1) of tRNA 711199
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.79D68A 59% of wild-type G(-1) addition activity. The mutation causes a dramatic decrease in the rigorous specificity of Thg1 for tRNAHis. This single alteration enhances the kcat/KM for ppp-tRNAPhe by nearly 100-fold relative to that of wild-type Thg1. G(-) i.e. the extra nucleotide posttranscriptionally added in position (-1) of tRNA 711199
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.79D77A less than 0.1% of wild-type G(-1) addition activity. G(-) i.e. the extra nucleotide posttranscriptionally added in position (-1) of tRNA 711199
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.79E13A 0.1%-1% of wild-type G(-1) addition activity. G(-) i.e. the extra nucleotide posttranscriptionally added in position (-1) of tRNA 711199
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.79E192A 2–3% of wild-type activity 711199
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.79E75A 105% of wild-type G(-1) addition activity. G(-) i.e. the extra nucleotide posttranscriptionally added in position (-1) of tRNA 711199
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.79E78A less than 0.1% of wild-type G(-1) addition activity. G(-) i.e. the extra nucleotide posttranscriptionally added in position (-1) of tRNA 711199
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.79G74A 44.4% of wild-type G(-1) addition activity. G(-) i.e. the extra nucleotide posttranscriptionally added in position (-1) of tRNA 711199
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