EC Number |
Protein Variants |
Reference |
---|
2.3.1.181 | C137A/C169A |
mutant protein retains trace activity |
661184 |
2.3.1.181 | C150A |
loss of the overall catalytic activity and an inability to form the acyl-enzyme intermediate |
711217 |
2.3.1.181 | C150S |
loss of the overall catalytic activity and an inability to form the acyl-enzyme intermediate |
711217 |
2.3.1.181 | C169A |
mutant enzyme retains trace activity, mutant enzyme forms an octanoyl-LpiB species that is not catalytically competent |
661184 |
2.3.1.181 | C169S |
mutant enzyme has no activity, mutant enzyme forms an octanoyl-LpiB species that is not catalytically competent |
661184 |
2.3.1.181 | C169S |
the hydrolyzed C169S mutant protein shows higher methyl octanoate levels than those of the wild type protein preparations |
712504 |
2.3.1.181 | K165A |
mutant has weakened catalytic ability |
711217 |
2.3.1.181 | K165R |
mutant remains active |
711217 |
2.3.1.181 | more |
construction of strain NM57 in which lipM is replaced with a kanamycin-resistance determinant. Gene lipM is placed under control of a xylose-inducible promoter PxylA is introduced into the DELTAlipM strain NM57 giving the lipM amyE::PxylA-lipM strain NM08. LipM can be functionally replaced by expression of Escherichia coli lipB, expression of Escherichia coli lipB allows growth of Bacillus subtilis DELTAlipL or DELATlipM strains in the absence of supplements. In contrast, growth of an Escherichia coli DELTAlipB strain can be complemented with lipM, but not lipL |
718133 |
2.3.1.181 | more |
Escherichia coli wild type strain and several spontaneous LipB knockout strains are used |
704311 |