EC Number   |
Protein Variants |
Reference |
|---|
   2.1.2.8 | C148G |
mutant is unable to catalyze a detectable 18O exchange in either the absence or presence of the folate cofactor |
485790 |
| 2.1.2.8 | C148S/D179N |
active site mutant |
757025 |
| 2.1.2.8 | D145N |
the mutant shows no activity |
757025 |
| 2.1.2.8 | D179A |
mutant not inactivated by 5-fluoro-dUMP |
485789 |
| 2.1.2.8 | D179N |
mutant with altered substrate preference, favoring dUMP rather than dCMP |
485789, 485790, 485792 |
| 2.1.2.8 | D179N |
mutation of active site, 10fold increased rate of inhibition by 5-fluoro-dUMP |
485789 |
| 2.1.2.8 | D179N |
the mutation significantly decreases the binding affinity to dCMP |
757025 |
| 2.1.2.8 | D179S |
mutant not inactivated by 5-fluoro-dUMP |
485789 |
| 2.1.2.8 | E60D |
mutant with decreased enzyme activity, impaired enzyme function in vitro and in vivo, uncoupling of early steps from later steps in catalysis |
485791 |
| 2.1.2.8 | E60Q |
mutant with decreased enzyme activity, impaired enzyme function in vitro and in vivo, uncoupling of early steps from later steps in catalysis |
485791 |
