Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Tequatrovirus T4 |
Crystallization (Comment) | Organism |
---|---|
C148S/D179N mutant in complex with dCMP and tetrahydrofolate, hanging drop vapor diffusion method, using 100 mM Tris-HCl pH 8.5 and 0.96 M sodium citrate | Tequatrovirus T4 |
Protein Variants | Comment | Organism |
---|---|---|
C148S/D179N | active site mutant | Tequatrovirus T4 |
D145N | the mutant shows no activity | Tequatrovirus T4 |
D179N | the mutation significantly decreases the binding affinity to dCMP | Tequatrovirus T4 |
S94A | the mutant 25% residual activity compared to the wild type enzyme | Tequatrovirus T4 |
S94N | the mutant shows no activity | Tequatrovirus T4 |
Y96F | the mutant shows no activity | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + dCMP | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyl-dCMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | P08773 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + dCMP | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyl-dCMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DCH | - |
Tequatrovirus T4 |
dCMP hydroxymethylase | - |
Tequatrovirus T4 |
deoxycytidylate hydroxymethylase | - |
Tequatrovirus T4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methenyltetrahydrofolate | - |
Tequatrovirus T4 |