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EC Number
Protein Variants
Commentary
Reference
2.1.1.33
D133A
complete loss of activity
757114
2.1.1.33
D133N
complete loss of activity
757114
2.1.1.33
D74A
21% of wild-type activity
757114
2.1.1.33
D97A
21% of wild-type activity
757114
2.1.1.33
E168A
100% of wild-type activity
757114
2.1.1.33
E47A
0.4% of wild-type activity
757114
2.1.1.33
Glu202Stop
is unstable to heat treatment as compared to the full-length wild-type enzyme, but has an enzymatic activity
676233
2.1.1.33
H104A
140% of wild-type activity
757114
2.1.1.33
K101A
36% of wild-type activity
757114
2.1.1.33
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combined knockdown of NSUN2,
EC 2.1.1.203
, and METTL1 in HeLa cells
737060
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