EC Number   |
Protein Variants |
Reference |
|---|
  2.1.1.190 | D265A |
the specific activity of the mutant enzyme is 830fold lower than that of the wild-type enzyme |
703145 |
| 2.1.1.190 | D265E |
mutant enzyme has no activity |
703145 |
| 2.1.1.190 | D363A |
mutation results in complete loss of activity |
703145 |
| 2.1.1.190 | D363N |
the specific activity of the mutant enzyme is 2fold lower than that of the wild-type enzyme |
703145 |
| 2.1.1.190 | D381A |
2% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 40% residual activity towards U1939 RNA substrate |
758197 |
| 2.1.1.190 | E424Q |
the mutant enzyme displays a biphasic reaction profile with an initial burst phase followed by a slow second phase, mutation results in at least 350fold reduction in the rate of proton abstraction |
703145 |
| 2.1.1.190 | F145A |
5% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 120% activity towards U1939 RNA substrate |
758197 |
| 2.1.1.190 | H151A |
5% residual activity towards U747 RNA substrate,reaction of EC 2.11.189, 55% activity towards U1939 RNA substrate |
758197 |
| 2.1.1.190 | N249A |
35% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 55% residual activity towards U1939 RNA substrate |
758197 |
| 2.1.1.190 | Q162A |
18% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 160% activity towards U1939 RNA substrate |
758197 |
