EC Number   |
Protein Variants |
Reference |
|---|
   1.8.1.B1 | C28A |
compared to the wild type enzyme, the mutant shows 38% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 4% activity with GSSG, and 74% activity with thioredoxin disulfide |
724324 |
| 1.8.1.B1 | C28A/C31A |
compared to the wild type enzyme, the mutant shows 50% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 3% activity with GSSG, and 79% activity with thioredoxin disulfide |
724324 |
| 1.8.1.B1 | C31A |
compared to the wild type enzyme, the mutant shows 38% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 18% activity with GSSG, and 100% activity with thioredoxin disulfide |
724324 |
| 1.8.1.B1 | C31S |
compared to the wild type enzyme, the mutant shows 100% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 129% activity with GSSG, and 105% activity with thioredoxin disulfide |
724324 |
| 1.8.1.B1 | C31S |
the mutant has negligible deglutathionylase and GR activities when compared with wild type enzyme |
725454 |
| 1.8.1.B1 | C34S |
both the deglutathionylase and GR activities of the mutant are only slightly lower when compared with wild type enzyme |
725454 |
| 1.8.1.B1 | C519S |
about 30% of wild-type activity with both substrates glutathione and 5,5'-dithio-bis(2-nitrobenzoic acid) |
741620 |
| 1.8.1.B1 | C519S |
the selenium content of wild-type enzyme and the C519S mutant enzyme is 17% and 15%, respectively |
763906 |
| 1.8.1.B1 | C520A |
compared to the wild type enzyme, the mutant shows 31% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 26% activity with GSSG, and 47% activity with thioredoxin disulfide |
724324 |
| 1.8.1.B1 | C520A/C574A |
compared to the wild type enzyme, the mutant shows 38% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 26% activity with GSSG, and 42% activity with thioredoxin disulfide |
724324 |
