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2-hydroxyethyl disulfide + NADPH + H+
?
-
-
-
-
?
5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
5'-thionitrobenzoic acid + NADP+
-
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
?
-
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
?
-
-
-
-
?
beta-hydroxyethyl disulfide + NADPH + H+
2 2-sulfanylethan-1-ol + NADP+
-
-
-
-
?
cytosolic thioredoxin + NADP+
cytosolic thioredoxin disulfide + NADPH
-
-
-
-
r
glutaredoxin + NADPH + H+
?
-
-
-
-
?
glutathione disulfide + NADPH + H+
2 glutathione + NADP+
glutathione disulfide + NADPH + H+
glutathione + NADP+
glutathione-hydroxyethyl disulfide + NADPH + H+
glutathione + 2-sulfanylethan-1-ol + NADP+
-
-
-
-
?
GSH + NADP+
GSSG + NADPH + H+
-
-
-
-
?
GSSG + NADPH + H+
glutathione + NADP+
GSSG + NADPH + H+
GSH + NADP+
hydroxyethyl disulfide + NADPH + H+
?
-
-
-
-
?
insulin disulfide + NADPH + H+
insulin + NADP+
-
-
-
-
?
mitochondrial thioredoxin + NADP+
mitochondrial thioredoxin disulfide + NADPH
-
-
-
-
r
oxidized insulin + NADPH + H+
reduced insulin + NADP+
-
-
-
-
?
thioredoxin + NADP+
thioredoxin disulfide + NADPH
-
-
-
-
?
thioredoxin + NADP+
thioredoxin disulfide + NADPH + H+
thioredoxin disulfide + NADPH
thioredoxin + NADP+
-
absolute specificity for NADPH as electron donor
-
-
?
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
additional information
?
-
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+

2-nitro-5-thiobenzoate + NADP+
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
-
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
-
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
-
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
-
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
-
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
-
absolute specificity for NADPH as electron donor
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
-
-
-
-
?
glutathione disulfide + NADPH + H+

2 glutathione + NADP+
-
enzyme displays hysteretic behavior
-
-
?
glutathione disulfide + NADPH + H+
2 glutathione + NADP+
-
-
-
-
?
glutathione disulfide + NADPH + H+
2 glutathione + NADP+
-
-
-
-
?
glutathione disulfide + NADPH + H+

glutathione + NADP+
-
-
-
-
?
glutathione disulfide + NADPH + H+
glutathione + NADP+
-
-
-
-
?
GSSG + NADPH + H+

glutathione + NADP+
-
-
-
-
?
GSSG + NADPH + H+
glutathione + NADP+
-
-
-
?
GSSG + NADPH + H+
glutathione + NADP+
-
absolute specificity for NADPH as electron donor
-
-
?
GSSG + NADPH + H+

GSH + NADP+
-
-
-
-
?
GSSG + NADPH + H+
GSH + NADP+
-
-
-
-
?
GSSG + NADPH + H+
GSH + NADP+
-
-
-
?
GSSG + NADPH + H+
GSH + NADP+
-
-
-
?
GSSG + NADPH + H+
GSH + NADP+
-
-
-
?
GSSG + NADPH + H+
GSH + NADP+
-
-
-
-
?
GSSG + NADPH + H+
GSH + NADP+
-
-
-
?
GSSG + NADPH + H+
GSH + NADP+
-
-
-
-
?
GSSG + NADPH + H+
GSH + NADP+
-
-
-
-
?
thioredoxin + NADP+

thioredoxin disulfide + NADPH + H+
-
-
-
-
?
thioredoxin + NADP+
thioredoxin disulfide + NADPH + H+
-
-
-
-
?
thioredoxin disulfide + NADPH + H+

thioredoxin + NADP+
-
-
-
-
?
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
-
-
-
?
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
-
-
-
-
?
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
-
-
-
?
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
-
-
-
-
?
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
-
-
-
?
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
-
-
-
-
?
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
-
-
-
-
?
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
-
-
-
?
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
-
-
-
-
?
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
-
Escherichia coli thioredoxin disulfide
-
-
?
additional information

?
-
-
the glutathione reductase activity of TGR exhibits hysteretic behavior regulated by the [GSSG]/[GSH] ratio. This behavior is associated with glutathionylation by GSSG and abolished by deglutathionylation
-
-
?
additional information
?
-
-
TGR promotes isomerization of disulfide bonds formed between glutathione peroxidase 4 and certain sperm proteins, generating a 46-kDa species containing glutathione peroxidase 4 from high molecular weight nonspecific cross-links
-
-
?
additional information
?
-
-
the parasite-specific enzyme thioredoxin glutathione reductase is a component of the defense system
-
-
?
additional information
?
-
-
low catalytic efficiency with H2O2
-
-
?
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(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis(2-furylmethanone)
-
-
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis(2-thienylmethanone)
-
-
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis[(1,3-dimethyl-1H-pyrazol-4-yl)methanone]
-
-
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis[(4-methoxyphenyl)methanone]
-
-
(3R,5R,8R) 5-(((3-carboxy-4-nitrophenyl)disulfanyl)methyl)tetrahydro-2H-thiazolo[4,3-b]thiazole-3-carboxylic acid
-
2-(4-chlorophenoxy)-6-methyl-2,3-dihydro-1,3,2-diazaphosphinin-4(1H)-one 2-oxide
-
-
2-(4-ethoxyphenoxy)-6-methyl-2,3-dihydro-1,3,2-diazaphosphinin-4(1H)-one 2-oxide
-
-
3-bromo-5-butoxy-6H-anthra[1,9-cd]isoxazol-6-one
-
-
3-DAP
-
0.05 mM, complete inhibition
4-chloro-5-[4-(hydroxymethyl)-1H-1,2,3-triazol-1-yl]-2-phenylpyridazin-3(2H)-one
-
at 0.010 microM, 100 % larval death at 48 hours
4-phenyl-1,2,5-oxadiazole-3-carbonitrile 2-oxide
-
-
5-(((3-carboxy-4-nitrophenyl)disulfanyl)methyl) (2RS,5RS)-3,3a-dihydro-benzo[d]thiazolo[4,3-b]thiazole
-
5-bromo-N-ethyl-3,4-dinitrothiophen-2-amine
-
at 0.005 microM, 100 % larval death at 48 hours
6-methyl-2-phenoxy-2,3-dihydro-1,3,2-diazaphosphinin-4(1H)-one 2-oxide
-
-
6-nitro-1H-1-benzothiophene-1,1-dione
-
at 0.010 microM, 100 % larval death at 48 hours
aurothioglucose
-
0.05 mM, complete inhibition
aurothiomalate
-
0.05 mM, complete inhibition
CDE16-2
-
0.05 mM, 98% inhibition
CDE4
-
0.05 mM, 99% inhibition
diethyl (3-bromo-6-oxo-6H-anthra[1,9-cd]isoxazol-5-yl)propanedioate
-
-
dimethyl (3-bromo-6-oxo-6H-anthra[1,9-cd]isoxazol-5-yl)propanedioate
-
-
JD159
-
0.05 mM, complete inhibition
LS826
-
0.05 mM, 95% inhibition
naphthazarin
-
0.05 mM, complete inhibition
NOC-7
-
i.e. 1-hydroxy-2-oxo-3-(N-3-methyl-aminopropyl)-3-methyl-1-triazene, complete inhibition at 0.1 mM
OPZ
-
0.05 mM, 87% inhibition
P-1,3-benzothiazol-2-yl-N-(2-fluorophenyl)-P-phenylphosphinic amide
-
-
P-1,3-benzothiazol-2-yl-N-(5-chloropyridin-2-yl)-P-phenylphosphinic amide
-
-
P-1,3-benzothiazol-2-yl-P-phenyl-N-1,3-thiazol-2-ylphosphinic amide
-
-
PAT
-
0.05 mM, complete inhibition
potassium antimony tartrate
-
-
RMA35
-
0.05 mM, 98% inhibition
[mu-[1-(hydroxy-1kappaO)-2-(sulfanyl-1kappaS)pyridin-1-iumato(2-)]]-[mu-[1-(hydroxy-2kappaO)-2-(sulfanyl-1kappaS)pyridin-1-iumato(2-)]]bis(triphenylphosphane)digold
-
a single gold atom binds to Cys519 and Cys573 in the AuI-enzyme complex. 75Ć¢ĀĀ80% inhibition of thioredoxin and glutathione reductase activities is achieved at a 1:1 concentration ratio of inhibitor:enzyme
additional information
-
the recombinant plasmid DNA vaccine pVAX1/SjTGR lowers enzymatic activity in vivo and in vitro
-
auranofin

-
the TGR inhibitor, killes larval worms in vitro
auranofin
-
auranofin at 0.005 mg/ml (0.0074 mM) reduces 57.1% of the thioredoxin reductase activity and 71% of the glutathione reductase activity in the Schistosoma japonicum adult worms
auranofin
very potent inhibitor, nearly compete inhibition at 0.01 mM
auranofin
-
0.05 mM, complete inhibition
auranofin
-
complete inhibition at 0.7 nM
auranofin
-
both cytolsolic and mitochondrial isoform
furoxan

-
-
furoxan
i.e. oxadiazole-3-carbonitrile-2-oxide, potent inhibitor
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2.792
2-hydroxyethyl disulfide
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
0.033 - 0.038
5,5'-dithio-bis(2-nitrobenzoic acid)
0.00434 - 0.71
5,5'-dithiobis(2-nitrobenzoic acid)
0.034
cytosolic thioredoxin
-
pH 7.0, 25ưC, wild-type enzyme
0.004 - 0.0612
glutathione disulfide
0.184 - 0.41
glutathione-hydroxyethyl disulfide
1.698
GSH
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
0.0095 - 0.012
mitochondial thioredoxin
0.0031 - 0.017
thioredoxin
0.00087 - 0.067
thioredoxin disulfide
additional information
additional information
-
kinetic properties of the Grx domain of TGR
-
0.033
5,5'-dithio-bis(2-nitrobenzoic acid)

-
mutant C574S, pH 7.0, temperature not specified in the publication
0.038
5,5'-dithio-bis(2-nitrobenzoic acid)
-
wild-type, pH 7.0, temperature not specified in the publication
0.034
5,5'-dithio-bis(2-nitrobenzoic acid)
-
mutant C519S, pH 7.0, temperature not specified in the publication
0.107
5,5'-dithiobis(2-nitrobenzoic acid)

-
mutant enzyme U597C, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.0345
5,5'-dithiobis(2-nitrobenzoic acid)
-
at pH 7.8 and 25ưC
0.043
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C31A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.045
5,5'-dithiobis(2-nitrobenzoic acid)
-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
0.049
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH 7.8, 25ưC
0.23
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C28A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.23
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.319
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.088
5,5'-dithiobis(2-nitrobenzoic acid)
-
cytosolic isoform, pH 7.8, temperature not specified in the publication
0.114
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH 7.4, 25ưC
0.1451
5,5'-dithiobis(2-nitrobenzoic acid)
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
0.169
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C31S, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.375
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C520A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.187
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C520A/C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.00434
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH 7.5, 25ưC
0.00434
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH not specified in the publication, temperature not specified in the publication
0.71
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
0.406
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C28A/C31A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.015
glutathione disulfide

-
wild-type, pH 7.0, temperature not specified in the publication
0.013
glutathione disulfide
-
mutant C574S, pH 7.0, temperature not specified in the publication
0.004
glutathione disulfide
-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
0.012
glutathione disulfide
-
mutant C519S, pH 7.0, temperature not specified in the publication
0.0612
glutathione disulfide
-
pH not specified in the publication, temperature not specified in the publication
0.0063
glutathione disulfide
-
cytosolic isoform, pH 7.8, temperature not specified in the publication
0.184
glutathione-hydroxyethyl disulfide

-
cytosolic isoform, pH 7.8, temperature not specified in the publication
0.41
glutathione-hydroxyethyl disulfide
-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
0.189
GSSG

-
mutant enzyme C31S, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.04955
GSSG
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
0.071
GSSG
-
mutant enzyme U597C, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.0715
GSSG
-
pH 7.4, 25ưC
0.042
GSSG
-
wild type enzyme, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.041
GSSG
-
at pH 7.8 and 25ưC
0.023
GSSG
-
mutant enzyme C28A/C31A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.036
GSSG
-
mutant enzyme C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.031
GSSG
-
mutant enzyme C520A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.024
GSSG
-
mutant enzyme C520A/C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.013
GSSG
-
mutant enzyme C31A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.015
GSSG
-
pH 7.8, 25ưC
0.016
GSSG
-
mutant enzyme C28A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.0095
mitochondial thioredoxin

-
pH 7.0, 25ưC, wild-type enzyme
0.012
mitochondial thioredoxin
-
pH 7.0, 25ưC, Sec-to-Cys mutant enzyme
0.0016
NADPH

-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
0.01097
NADPH
-
with glutaredoxin as cosubstrate, at 25ưC in 0.1 M potassium phosphate (pH 7.4)
0.0137
NADPH
-
pH 7.4, 25ưC
0.08324
NADPH
-
with GSSG as cosubstrate, at 25ưC in 0.1 M potassium phosphate (pH 7.4)
0.019
NADPH
-
at pH 7.8 and 25ưC
0.02142
NADPH
-
with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, at 25ưC in 0.1 M potassium phosphate (pH 7.4)
0.048
NADPH
-
cytosolic isoform, pH 7.8, temperature not specified in the publication
0.003
NADPH
-
pH 7.8, 25ưC
0.017
thioredoxin

-
pH 7.8, 25ưC
0.0031
thioredoxin
-
pH 7.8, temperature not specified in the publication
0.00087
thioredoxin disulfide

-
at pH 7.8 and 25ưC
0.067
thioredoxin disulfide
-
cytosolic isoform, pH 7.8, temperature not specified in the publication
0.022
thioredoxin disulfide
-
mutant enzyme C28A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.019
thioredoxin disulfide
-
mutant enzyme C28A/C31A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.015
thioredoxin disulfide
-
mutant enzyme U597C, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.015
thioredoxin disulfide
-
mutant enzyme C520A/C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.008
thioredoxin disulfide
-
wild type enzyme, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.008
thioredoxin disulfide
-
mutant enzyme C31S, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.008
thioredoxin disulfide
-
mutant enzyme C31A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.007
thioredoxin disulfide
-
mutant enzyme C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.007
thioredoxin disulfide
-
mutant enzyme C520A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.00324
thioredoxin disulfide
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8.65
2-hydroxyethyl disulfide
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
10.2 - 55
5,5'-dithio-bis(2-nitrobenzoic acid)
0.17 - 118
5,5'-dithiobis(2-nitrobenzoic acid)
197
cytosolic thioredoxin
-
pH 7.0, 25ưC, wild-type enzyme
0.96 - 8
glutathione disulfide
5.13 - 163.5
glutathione-hydroxyethyl disulfide
15.78
GSH
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
6.4 - 131
mitochondial thioredoxin
0.35 - 9
thioredoxin disulfide
55
5,5'-dithio-bis(2-nitrobenzoic acid)

-
wild-type, pH 7.0, temperature not specified in the publication
12
5,5'-dithio-bis(2-nitrobenzoic acid)
-
mutant C519S, pH 7.0, temperature not specified in the publication
10.2
5,5'-dithio-bis(2-nitrobenzoic acid)
-
mutant C574S, pH 7.0, temperature not specified in the publication
6
5,5'-dithiobis(2-nitrobenzoic acid)

-
mutant enzyme C28A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
5
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C520A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
6
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C31A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
6
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C520A/C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
6
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
4
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme U597C, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
8
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C28A/C31A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
8.16
5,5'-dithiobis(2-nitrobenzoic acid)
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
1.85
5,5'-dithiobis(2-nitrobenzoic acid)
-
cytosolic isoform, pH 7.8, temperature not specified in the publication
1.2
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
12.6
5,5'-dithiobis(2-nitrobenzoic acid)
-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
16
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH 7.4, 25ưC
16
5,5'-dithiobis(2-nitrobenzoic acid)
-
mutant enzyme C31S, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
16
5,5'-dithiobis(2-nitrobenzoic acid)
-
wild type enzyme, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
118
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH 7.0, 25ưC, wild-type enzyme
0.3
5,5'-dithiobis(2-nitrobenzoic acid)
-
at pH 7.8 and 25ưC
0.17
5,5'-dithiobis(2-nitrobenzoic acid)
-
pH 7.8, 25ưC
5
glutathione disulfide

-
mutant C574S, pH 7.0, temperature not specified in the publication
5.6
glutathione disulfide
-
mutant C519S, pH 7.0, temperature not specified in the publication
0.96
glutathione disulfide
-
cytosolic isoform, pH 7.8, temperature not specified in the publication
1.62
glutathione disulfide
-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
2 - 8
glutathione disulfide
-
wild-type, pH 7.0, temperature not specified in the publication
5.13
glutathione-hydroxyethyl disulfide

-
cytosolic isoform, pH 7.8, temperature not specified in the publication
163.5
glutathione-hydroxyethyl disulfide
-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
0.19
GSSG

-
-
5
GSSG
-
mutant enzyme C520A/C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
25
GSSG
-
mutant enzyme C31S, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
21.7
GSSG
-
pH 7.4, 25ưC
19.4
GSSG
-
wild type enzyme, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.6
GSSG
-
mutant enzyme C28A/C31A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.7
GSSG
-
mutant enzyme C28A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.84
GSSG
-
at pH 7.8 and 25ưC
5.1
GSSG
-
pH 7.8, 25ưC
5.35
GSSG
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
5
GSSG
-
mutant enzyme C520A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
4
GSSG
-
mutant enzyme U597C, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
3.5
GSSG
-
mutant enzyme C31A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
7
GSSG
-
mutant enzyme C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
6.4
mitochondial thioredoxin

-
pH 7.0, 25ưC, Sec-to-Cys mutant enzyme
131
mitochondial thioredoxin
-
pH 7.0, 25ưC, wild-type enzyme
14.93
NADPH

-
with glutaredoxin as cosubstrate, at 25ưC in 0.1 M potassium phosphate (pH 7.4)
12.6
NADPH
-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
20.1
NADPH
-
pH 7.4, 25ưC
4.87
NADPH
-
with GSSG as cosubstrate, at 25ưC in 0.1 M potassium phosphate (pH 7.4)
10.92
NADPH
-
with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, at 25ưC in 0.1 M potassium phosphate (pH 7.4)
1.64
NADPH
-
cytosolic isoform, pH 7.8, temperature not specified in the publication
19.2
thioredoxin

-
pH 7.8, 25ưC
10
thioredoxin
-
pH 7.8, temperature not specified in the publication
3.5
thioredoxin disulfide

-
mutant enzyme U597C, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
8
thioredoxin disulfide
-
mutant enzyme C520A/C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
8
thioredoxin disulfide
-
mutant enzyme C574A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
4.78
thioredoxin disulfide
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
0.47
thioredoxin disulfide
-
at pH 7.8 and 25ưC
0.35
thioredoxin disulfide
-
cytosolic isoform, pH 7.8, temperature not specified in the publication
9
thioredoxin disulfide
-
mutant enzyme C520A, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3.1
2-hydroxyethyl disulfide
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
0.31 - 1.45
5,5'-dithio-bis(2-nitrobenzoic acid)
8.7 - 2800
5,5'-dithiobis(2-nitrobenzoic acid)
0.38 - 420
glutathione disulfide
28 - 390
glutathione-hydroxyethyl disulfide
9.3
GSH
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
5.2 - 1480
thioredoxin disulfide
0.35
5,5'-dithio-bis(2-nitrobenzoic acid)

-
mutant C519S, pH 7.0, temperature not specified in the publication
1.45
5,5'-dithio-bis(2-nitrobenzoic acid)
-
wild-type, pH 7.0, temperature not specified in the publication
0.31
5,5'-dithio-bis(2-nitrobenzoic acid)
-
mutant C574S, pH 7.0, temperature not specified in the publication
21
5,5'-dithiobis(2-nitrobenzoic acid)

-
cytosolic isoform, pH 7.8, temperature not specified in the publication
119
5,5'-dithiobis(2-nitrobenzoic acid)
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
2800
5,5'-dithiobis(2-nitrobenzoic acid)
-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
8.7
5,5'-dithiobis(2-nitrobenzoic acid)
-
at pH 7.8 and 25ưC
1.87
glutathione disulfide

-
wild-type, pH 7.0, temperature not specified in the publication
0.47
glutathione disulfide
-
mutant C519S, pH 7.0, temperature not specified in the publication
0.38
glutathione disulfide
-
mutant C574S, pH 7.0, temperature not specified in the publication
150
glutathione disulfide
-
cytosolic isoform, pH 7.8, temperature not specified in the publication
420
glutathione disulfide
-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
28
glutathione-hydroxyethyl disulfide

-
cytosolic isoform, pH 7.8, temperature not specified in the publication
390
glutathione-hydroxyethyl disulfide
-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
22
GSSG

-
at pH 7.8 and 25ưC
108
GSSG
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
7900
NADPH

-
mitochondrial isoform, pH 7.8, temperature not specified in the publication
1360
NADPH
-
with glutaredoxin as cosubstrate, at 25ưC in 0.1 M potassium phosphate (pH 7.4)
510
NADPH
-
with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, at 25ưC in 0.1 M potassium phosphate (pH 7.4)
58.5
NADPH
-
with GSSG as cosubstrate, at 25ưC in 0.1 M potassium phosphate (pH 7.4)
34
NADPH
-
cytosolic isoform, pH 7.8, temperature not specified in the publication
540
thioredoxin disulfide

-
at pH 7.8 and 25ưC
1480
thioredoxin disulfide
-
at 25ưC in 0.1 M potassium phosphate (pH 7.4)
5.2
thioredoxin disulfide
-
cytosolic isoform, pH 7.8, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.000025 - 0.000082
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis(2-furylmethanone)
0.00002 - 0.00051
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis(2-thienylmethanone)
0.00129 - 0.00408
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis[(1,3-dimethyl-1H-pyrazol-4-yl)methanone]
0.00006 - 0.00244
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis[(4-methoxyphenyl)methanone]
0.024
(3R,5R,8R) 5-(((3-carboxy-4-nitrophenyl)disulfanyl)methyl)tetrahydro-2H-thiazolo[4,3-b]thiazole-3-carboxylic acid
Echinococcus granulosus
pH 7.0, 25ưC
0.00055
2-(4-chlorophenoxy)-6-methyl-2,3-dihydro-1,3,2-diazaphosphinin-4(1H)-one 2-oxide
Schistosoma mansoni
-
-
0.0006
2-(4-ethoxyphenoxy)-6-methyl-2,3-dihydro-1,3,2-diazaphosphinin-4(1H)-one 2-oxide
Schistosoma mansoni
-
-
0.00015
3-bromo-5-butoxy-6H-anthra[1,9-cd]isoxazol-6-one
Schistosoma mansoni
-
-
0.0063
4-chloro-5-[4-(hydroxymethyl)-1H-1,2,3-triazol-1-yl]-2-phenylpyridazin-3(2H)-one
Schistosoma mansoni
-
pH 7.5, 22ưC
0.00032 - 0.0018
4-phenyl-1,2,5-oxadiazole-3-carbonitrile 2-oxide
0.014
5-(((3-carboxy-4-nitrophenyl)disulfanyl)methyl) (2RS,5RS)-3,3a-dihydro-benzo[d]thiazolo[4,3-b]thiazole
Echinococcus granulosus
pH 7.0, 25ưC
3.15
5-bromo-N-ethyl-3,4-dinitrothiophen-2-amine
Schistosoma mansoni
-
pH 7.5, 22ưC
0.004
6-methyl-2-phenoxy-2,3-dihydro-1,3,2-diazaphosphinin-4(1H)-one 2-oxide
Schistosoma mansoni
-
-
0.0054
6-nitro-1H-1-benzothiophene-1,1-dione
Schistosoma mansoni
-
pH 7.5, 22ưC
0.0000032 - 0.00001
auranofin
0.00007 - 0.003
aurothioglucose
0.00005 - 0.00009
aurothiomalate
0.009
diethyl (3-bromo-6-oxo-6H-anthra[1,9-cd]isoxazol-5-yl)propanedioate
Schistosoma mansoni
-
-
0.00008
dimethyl (3-bromo-6-oxo-6H-anthra[1,9-cd]isoxazol-5-yl)propanedioate
Schistosoma mansoni
-
-
0.0025 - 0.01
naphthazarin
0.012 - 0.0203
P-1,3-benzothiazol-2-yl-N-(2-fluorophenyl)-P-phenylphosphinic amide
0.000789 - 0.00154
P-1,3-benzothiazol-2-yl-N-(5-chloropyridin-2-yl)-P-phenylphosphinic amide
0.0000212 - 0.000121
P-1,3-benzothiazol-2-yl-P-phenyl-N-1,3-thiazol-2-ylphosphinic amide
0.000008 - 0.000045
potassium antimony tartrate
0.000038
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis(2-furylmethanone)

Schistosoma mansoni
-
pH 7, thioredoxin reductase activity
0.000082
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis(2-furylmethanone)
Schistosoma mansoni
-
pH 7, glutathione reductase activity
0.000025
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis(2-furylmethanone)
Schistosoma mansoni
-
-
0.000042
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis(2-thienylmethanone)

Schistosoma mansoni
-
pH 7, thioredoxin reductase activity
0.00051
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis(2-thienylmethanone)
Schistosoma mansoni
-
pH 7, glutathione reductase activity
0.00002
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis(2-thienylmethanone)
Schistosoma mansoni
-
-
0.002
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis[(1,3-dimethyl-1H-pyrazol-4-yl)methanone]

Schistosoma mansoni
-
-
0.00408
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis[(1,3-dimethyl-1H-pyrazol-4-yl)methanone]
Schistosoma mansoni
-
pH 7, glutathione reductase activity
0.00129
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis[(1,3-dimethyl-1H-pyrazol-4-yl)methanone]
Schistosoma mansoni
-
pH 7, thioredoxin reductase activity
0.000105
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis[(4-methoxyphenyl)methanone]

Schistosoma mansoni
-
pH 7, thioredoxin reductase activity
0.00244
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis[(4-methoxyphenyl)methanone]
Schistosoma mansoni
-
pH 7, glutathione reductase activity
0.00006
(2-oxido-1,2,5-oxadiazole-3,4-diyl)bis[(4-methoxyphenyl)methanone]
Schistosoma mansoni
-
-
0.007
3-DAP

Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.0005
3-DAP
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
0.0018
4-phenyl-1,2,5-oxadiazole-3-carbonitrile 2-oxide

Schistosoma mansoni
-
-
0.00167
4-phenyl-1,2,5-oxadiazole-3-carbonitrile 2-oxide
Schistosoma mansoni
-
pH 7, thioredoxin reductase activity
0.00032
4-phenyl-1,2,5-oxadiazole-3-carbonitrile 2-oxide
Schistosoma mansoni
-
pH 7, glutathione reductase activity
0.0000054
auranofin

Schistosoma mansoni
-
wild type enzyme, with GSSG as substrate, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.0000032
auranofin
Schistosoma mansoni
-
wild type enzyme, with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.000007
auranofin
Schistosoma mansoni
pH 7.0, 25ưC, thioredoxin reductase activity
0.000007
auranofin
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
0.0000083
auranofin
Schistosoma mansoni
-
wild type enzyme, with thioredoxin disulfide as substrate, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.000009
auranofin
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.00001
auranofin
Schistosoma mansoni
pH 7.0, 37ưC, glutathione reductase activity
0.00007
aurothioglucose

Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
0.003
aurothioglucose
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.00009
aurothiomalate

Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
0.00005
aurothiomalate
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.001
CDE16-2

Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.025
CDE16-2
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
0.0005
CDE4

Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.007
CDE4
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
0.003
furoxan

Schistosoma mansoni
-
wild type enzyme, with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.0032
furoxan
Schistosoma mansoni
-
wild type enzyme, with GSSG as substrate, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.0054
furoxan
Schistosoma mansoni
-
wild type enzyme, with thioredoxin disulfide as substrate, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.0005
JD159

Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
0.001
JD159
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.008
LS826

Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.008
LS826
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
0.0025
naphthazarin

Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
0.01
naphthazarin
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.08
OPZ

Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
0.05
OPZ
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.012
P-1,3-benzothiazol-2-yl-N-(2-fluorophenyl)-P-phenylphosphinic amide

Schistosoma mansoni
-
-
0.0151
P-1,3-benzothiazol-2-yl-N-(2-fluorophenyl)-P-phenylphosphinic amide
Schistosoma mansoni
-
pH 7, thioredoxin reductase activity
0.0203
P-1,3-benzothiazol-2-yl-N-(2-fluorophenyl)-P-phenylphosphinic amide
Schistosoma mansoni
-
pH 7, glutathione reductase activity
0.00154
P-1,3-benzothiazol-2-yl-N-(5-chloropyridin-2-yl)-P-phenylphosphinic amide

Schistosoma mansoni
-
pH 7, glutathione reductase activity
0.000789
P-1,3-benzothiazol-2-yl-N-(5-chloropyridin-2-yl)-P-phenylphosphinic amide
Schistosoma mansoni
-
pH 7, thioredoxin reductase activity
0.0008
P-1,3-benzothiazol-2-yl-N-(5-chloropyridin-2-yl)-P-phenylphosphinic amide
Schistosoma mansoni
-
-
0.0000212
P-1,3-benzothiazol-2-yl-P-phenyl-N-1,3-thiazol-2-ylphosphinic amide

Schistosoma mansoni
-
pH 7, thioredoxin reductase activity
0.000025
P-1,3-benzothiazol-2-yl-P-phenyl-N-1,3-thiazol-2-ylphosphinic amide
Schistosoma mansoni
-
-
0.000121
P-1,3-benzothiazol-2-yl-P-phenyl-N-1,3-thiazol-2-ylphosphinic amide
Schistosoma mansoni
-
pH 7, glutathione reductase activity
0.000051
PAT

Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
0.000037
PAT
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.000008
potassium antimony tartrate

Schistosoma mansoni
-
wild type enzyme, with GSSG as substrate, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.000009
potassium antimony tartrate
Schistosoma mansoni
-
wild type enzyme, with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.000045
potassium antimony tartrate
Schistosoma mansoni
-
wild type enzyme, with thioredoxin disulfide as substrate, in 0.1 M potassium phosphate buffer and 10 mM EDTA (pH 7.4) at 25ưC
0.006
RMA35

Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: GSSG + NADPH
0.004
RMA35
Schistosoma mansoni
-
pH 7.4, 25ưC, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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H464Q
-
mutant enzyme has only 2% of the wild-type activity. The pH dependence of Vmax for wild-type DmTrxR has pKa values of 6.4 and 9.3 on the DmTrxR-DmTrx-2 complex, whereas H464Q DmTrxR only has an observable pKa at 6.4, indicating that the pKa at pH 9.3 is contributed mainly by His464. The pKa at pH 6.4 is assigned to Cys57 and Cys490. The thiolate on Cys490 is the nucleophile in the reduction of Trx. In contrast to wild-type DmTrxR, H464Q DmTrxR does not stabilize a thiolate-FAD charge-transfer complex in the presence of excess NADPH. The rates of steps in both the reductive and the oxidative half-reactions are markedly diminished in H464Q DmTrxR as compared to those of wild-type enzyme, indicating that His464 is involved in both half-reactions
C31S
-
the mutant has negligible deglutathionylase and GR activities when compared with wild type enzyme
C34S
-
both the deglutathionylase and GR activities of the mutant are only slightly lower when compared with wild type enzyme
C519S
-
about 30% of wild-type activity with both substrates glutathione and 5,5'-dithio-bis(2-nitrobenzoic acid)
C574S
-
about 30% of wild-type activity with both substrates glutathione and 5,5'-dithio-bis(2-nitrobenzoic acid)
C28A
-
compared to the wild type enzyme, the mutant shows 38% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 4% activity with GSSG, and 74% activity with thioredoxin disulfide
C28A/C31A
-
compared to the wild type enzyme, the mutant shows 50% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 3% activity with GSSG, and 79% activity with thioredoxin disulfide
C31A
-
compared to the wild type enzyme, the mutant shows 38% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 18% activity with GSSG, and 100% activity with thioredoxin disulfide
C31S
-
compared to the wild type enzyme, the mutant shows 100% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 129% activity with GSSG, and 105% activity with thioredoxin disulfide
C520A
-
compared to the wild type enzyme, the mutant shows 31% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 26% activity with GSSG, and 47% activity with thioredoxin disulfide
C520A/C574A
-
compared to the wild type enzyme, the mutant shows 38% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 26% activity with GSSG, and 42% activity with thioredoxin disulfide
C574A
-
compared to the wild type enzyme, the mutant shows 38% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 37% activity with GSSG, and 42% activity with thioredoxin disulfide
additional information
-
analysis of TGR mutants reveales that the glutaredoxin domain is required for the glutathione reductase activity but does not affect the thioredoxin reductase activity. In contrast, both glutathione reductase and thioredoxin reductase activities are dependent on the Sec-containing redox center. The activity loss caused by the Sec-to-Cys mutation can be partially compensated by a Cys-to-Sec mutation of the neighboring residue, indicating that Sec can support catalysis at this alternative position
U597C

-
compared to the wild type enzyme, the mutant shows 25% activity with 5,5'-dithiobis(2-nitrobenzoic acid), 21% activity with GSSG, and 18% activity with thioredoxin disulfide
U597C
the mutant displays significantly decreased enzyme activities compared to the wild type enzyme
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Taenia crassiceps
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Schistosoma mansoni
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