EC Number   |
Protein Variants |
Reference |
|---|
    1.2.1.105 | D114A |
mutation in component E1, a 6fold decrease in sensitivity for Ca2+ |
758747 |
| 1.2.1.105 | H260A |
mutation drastically reduces catalytic activity |
758781 |
| 1.2.1.105 | H260E |
mutation drastically reduces catalytic activity |
758781 |
| 1.2.1.105 | H260E/H298N |
almost 20fold decrease in kcat/Km vlaue |
758781 |
| 1.2.1.105 | H298A |
mutation drastically reduces catalytic activity |
758781 |
| 1.2.1.105 | H298D |
mutant accepts unnatural substrate 2-oxovalerate |
758781 |
| 1.2.1.105 | H298L |
mutant accepts unnatural substrate 2-oxovalerate |
758781 |
| 1.2.1.105 | H298T |
mutant accepts unnatural substrate 2-oxovalerate |
758781 |
| 1.2.1.105 | H298V |
mutant accepts unnatural substrate 2-oxovalerate |
758781 |
| 1.2.1.105 | more |
component E1 has two potential Ca2+ -binding sites, site 1 (D114ADLD) and site 2 (E139SDLD). A variant in site 1 (sequence of site 1 is replaced by A114AALA) exhibits an almost complete loss of Ca2+ activation. A variant in site 2 (sequence is replaced with A139SALA) shows no measurable change in Ca2+ sensitivity |
758747 |
