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Literature summary for 1.2.1.105 extracted from

  • Armstrong, C.T.; Anderson, J.L.; Denton, R.M.
    Studies on the regulation of the human E1 subunit of the 2-oxoglutarate dehydrogenase complex, including the identification of a novel calcium-binding site (2014), Biochem. J., 459, 369-381 .
    View publication on PubMed
Search for more literature for 1.2.1.105

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sus scrofa
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
D114A mutation in component E1, a 6fold decrease in sensitivity for Ca2+ Sus scrofa
D114A mutation in component E1, a 6fold decrease in sensitivity for Ca2+ Homo sapiens
additional information component E1 has two potential Ca2+ -binding sites, site 1 (D114ADLD) and site 2 (E139SDLD). A variant in site 1 (sequence of site 1 is replaced by A114AALA) exhibits an almost complete loss of Ca2+ activation. A variant in site 2 (sequence is replaced with A139SALA) shows no measurable change in Ca2+ sensitivity Sus scrofa
additional information component E1 has two potential Ca2+ -binding sites, site 1 (D114ADLD) and site 2 (E139SDLD). A variant in site 1 (sequence of site 1 is replaced by A114AALA) exhibits an almost complete loss of Ca2+ activation. A variant in site 2 (sequence is replaced with A139SALA) shows no measurable change in Ca2+ sensitivity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 2-oxoglutarate overall activity of the complex, presence of Ca2+, absence of NADH, 30°C, pH not specified in the publication Sus scrofa
0.08
-
 2-oxoglutarate activity of component E1, presence of Ca2+, absence of NADH, 30°C, pH not specified in the publication Sus scrofa
0.11
-
 2-oxoglutarate overall activity of the complex, presence of Ca2+, presence of NADH, 30°C, pH not specified in the publication Sus scrofa
0.13
-
 2-oxoglutarate activity of component E1, absence of Ca2+, absence of NADH, 30°C, pH not specified in the publication Sus scrofa
0.15
-
 2-oxoglutarate activity of component E1, presence of Ca2+, absence of NADH, 30°C, pH not specified in the publication Homo sapiens
0.2
-
 2-oxoglutarate activity of component E1, presence of Ca2+, presence of NADH, 30°C, pH not specified in the publication Homo sapiens
0.21
-
 2-oxoglutarate activity of component E1, presence of Ca2+, presence of NADH, 30°C, pH not specified in the publication Sus scrofa
0.28
-
 2-oxoglutarate activity of component E1, absence of Ca2+, absence of NADH, 30°C, pH not specified in the publication Homo sapiens
1.28
-
 2-oxoglutarate overall activity of the complex, absence of Ca2+, absence of NADH, 30°C, pH not specified in the publication Sus scrofa
1.48
-
 2-oxoglutarate activity of component E1, absence of Ca2+, presence of NADH, 30°C, pH not specified in the publication Sus scrofa
1.57
-
 2-oxoglutarate activity of component E1, absence of Ca2+, presence of NADH, 30°C, pH not specified in the publication Homo sapiens
10.1
-
 2-oxoglutarate overall activity of the complex, absence of Ca2+, presence of NADH, 30°C, pH not specified in the publication Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Sus scrofa
Ca2+ K0.5 value in absence of NADH is 0.00013 mM, in presence of NADH is 0.00781 mM. Component E1 has two potential Ca2+ -binding sites, site 1 (D114ADLD) and site 2 (E139SDLD). A mutated form of E1, D114A, shows a 6-fold decrease in sensitivity for Ca2+ ,whereas variantx02site1 (sequence of site 1 is replaced by A114AALA) exhibits an almost complete loss of Ca2+ activation. A variant in site2 (sequence is replaced with A139SALA) shows no measurable change in Ca2+ sensitivity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q02218 i.e. ODGH, component E1 of the complex, cf. EC 1.2.4.2
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + CoA + NAD+
-
 Sus scrofa succinyl-CoA + CO2 + NADH
-
 ?
2-oxoglutarate + CoA + NAD+
-
 Homo sapiens succinyl-CoA + CO2 + NADH
-
 ?

Subunits

Subunits Comment Organism
? x * 110000, SDS-PAGE of recombinant component E1 Sus scrofa
? x * 110000, SDS-PAGE of recombinant component E1 Homo sapiens

Synonyms

Synonyms Comment Organism
ODGH
-
 Sus scrofa
ODGH
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
CoA
-
 Sus scrofa
CoA
-
 Homo sapiens
NAD+
-
 Sus scrofa
NAD+
-
 Homo sapiens
thiamine diphosphate
-
 Sus scrofa
thiamine diphosphate
-
 Homo sapiens