EC Number   |
Protein Variants |
Reference |
|---|
    5.3.2.6 | P1A |
inactive |
-, 749336 |
| 5.3.2.6 | alphaL8R/I52E |
site-directed mutagenesis, active site mutant, the mutant shows improved trans-3-chloroacrylic acid dehalogenase activity with a 32fold increase in kcat/Km, largely due to a 23fold decrease in Km, and diminished 4-oxalocrotonate tautomerase activity with a 1700fold decrease in kcat/Km |
-, 718844 |
| 5.3.2.6 | alphaL8R |
site-directed mutagenesis, active site mutant, the mutant shows improved trans-3-chloroacrylic acid dehalogenase activity with a 50fold increase in kcat/Km, primarily from an 8.8fold increase in kcat, and diminished 4-oxalocrotonate tautomerase activity with a 5fold decrease in kcat/Km. The increased CaaD activity of L8R-4-OT does not substantially diminish the original 4-OT activity |
-, 718844 |
| 5.3.2.6 | alphaI52E |
site-directed mutagenesis, active site mutant,the mutant shows improved trans-3-chloroacrylic acid dehalogenase activity with a 36fold increase in kcat/Km, largely due to a 110fold decrease in Km, and diminished 4-oxalocrotonate tautomerase activity. The negatively charged group may hinder the formation of the enolate intermediate and may contribute to a decrease in kcat |
-, 718844 |
| 5.3.2.6 | R61A |
site-directed mutagenesis, Arg61 mutation does not affect either substrate binding or catalysis |
-, 718830 |
| 5.3.2.6 | P1A |
site-directed mutagenesis, inactive mutant |
718847 |
| 5.3.2.6 | R11A |
site-directed mutagenesis, inactive mutant |
718847 |
| 5.3.2.6 | R11A/R39A |
site-directed mutagenesis, inactive mutant |
-, 718830 |
| 5.3.2.6 | R11A |
site-directed mutagenesis, no kinetic effects of the R11A mutation, the stereoselectivity of the R11A-catalyzed protonation at C-5 of the dicarboxylate substrate decreases, while the stereoselectivity of protonation at C-3 of the monocarboxylate substrate increases in comparison with wild-type 4-OT |
-, 718830 |
| 5.3.2.6 | alphaR12A |
site-directed mutagenesis, the mutant shows highly reduced catalytic efficiency compared to the wild-type hh4-OT |
-, 718874 |
