EC Number   |
Protein Variants |
Reference |
|---|
   4.1.3.43 | G323L |
63% channeling efficiency for acetaldehyde, unable to channel propionaldehyde |
721651 |
| 4.1.3.43 | more |
all mutations created by site specific mutagenesis |
721651 |
| 4.1.3.43 | G322A |
channels acetaldehyde with similar efficiency to wild-type, 30% lowered efficiency of isobutyraldehyde channeling |
721651 |
| 4.1.3.43 | G323A |
channels acetaldehyde with similar efficiency to wild-type, unable to channel isobutyraldehyde |
721651 |
| 4.1.3.43 | L87N/Y290F |
exhibits stereoselectivity opposite to that of the wild type and alkyl chain length of the aldehyde does not affect stereochemical control |
722458 |
| 4.1.3.43 | L87W/Y290F |
exhibits stereoselectivity opposite to that of the wild type and alkyl chain length of the aldehyde does not affect stereochemical control |
722458 |
| 4.1.3.43 | A324G |
increase of channeling efficiency of propionaldehyde to a value comparable to that of acetaldehyde |
721659 |
| 4.1.3.43 | L87N |
mutant created by site-specific mutagenesis |
722458 |
| 4.1.3.43 | L87W |
mutant created by site-specific mutagenesis |
722458 |
| 4.1.3.43 | Y290F |
mutant generated by site-directed mutagenesis, mutation results in a loss of stereochemical control as the variant is able to utilize substrates with R and S configurations at C4 with similar kinetic parameters |
714244 |
