EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.30 | K130I |
mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type |
637629 |
| 2.5.1.30 | L94S |
9fold lower Vmax values and 7fold higher Km-values for the allylic substrate farnesyl diphosphate compared to wild-type enzyme |
637629 |
| 2.5.1.30 | more |
elucidation of the mechanism of chain length determination of Bacillus stearothermophilus HepPS by site-directed mutagenesis |
637630 |
| 2.5.1.30 | more |
several amino acid residues in the larger subunits Bacillus subtilis heptaprenyl diphosphate synthase are selected for substitutions by site-directed mutagenesis and examined by combination with the corresponding wild type or mutated smaller subunits |
702201 |
| 2.5.1.30 | more |
the hybrid-type combination of component I (Bacillus subtilis) and component II (Bacillus stearothermophilus) gives distinct prenyltransferase activity. The hybrid-type enzyme catalyzes the synthesis of heptaprenyl diphosphate and shows moderate heat stability, which is between those of the natural enzymes from Bacillus subtilis and Bacillus stearothermophilus. There is no possibility of forming a hybrid between the heptaprenyl and hexaprenyl diphosphate synthases |
637628 |
| 2.5.1.30 | more |
the hybrid-type combination of component I (Bacillus subtilis) and component II' (Bacillus stearothermophilus) gives distinct prenyltransferase activity. The hybrid-type enzyme catalyzes the synthesis of heptaprenyl diphosphate and shows moderate heat stability, which is between those of the natural enzymes from Bacillus subtilis and Bacillus stearothermophilus |
637631 |
| 2.5.1.30 | N127A |
mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type |
637629 |
| 2.5.1.30 | S80F |
single replacement to the aromatic residue at the fourth or the fifth position before the first aspartate-rich motif (FARM), mainly yields a C20 product |
637630 |
| 2.5.1.30 | T76V |
mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type |
637629 |
| 2.5.1.30 | V76I |
mutant enzyme subunit-I(wild-type)/subunit-II(A79L), octaprenyl diphosphate is the final product with farnesyl diphosphate as allylic primer |
702201 |
