EC Number |
Protein Variants |
Reference |
---|
3.4.22.10 | G163S/A172S |
protease activity (microgram/min/mg) substrate azocasein: 286, substrate proSPE B C47S mutant: 236, compared to wild-type 341 and 378, respectively |
698880 |
3.4.22.10 | G239D |
protease activity (microgram/min/mg) substrate azocasein: 35.3, substrate proSPE B C47S mutant: 20, compared to wild-type 341 and 378, respectively |
698880 |
3.4.22.10 | G308S |
behaviour is similar to the wild type enzyme |
680146 |
3.4.22.10 | G378A |
Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type |
-, 734185 |
3.4.22.10 | G380A |
Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type |
-, 734185 |
3.4.22.10 | G381A |
Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type |
-, 734185 |
3.4.22.10 | G382A |
Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type |
-, 734185 |
3.4.22.10 | G384A |
Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type |
734185 |
3.4.22.10 | G384D |
Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) 50% decreased compared to wild-type |
-, 734185 |
3.4.22.10 | G385A |
Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) minimally increased compared to wild-type and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) 3fold increased |
734185 |