EC Number |
Protein Variants |
Reference |
---|
3.4.22.10 | more |
construction of an isogenic inactive mutant strain M6 |
670124 |
3.4.22.10 | T379A |
Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) slightly decreased compared to wild-type |
734185 |
3.4.22.10 | T379V |
Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) slightly decreased compared to wild-type |
734185 |
3.4.22.10 | V189A |
protease activity (microgram/min/mg) substrate azocasein: 2.6, substrate proSPE B C47S mutant: 0.9, compared to wild-type 341 and 378, respectively |
698880 |
3.4.22.10 | W212A |
protease activity (microgram/min/mg) substrate azocasein: 1.2, substrate proSPE B C47S mutant: 0.9, compared to wild-type 341 and 378, respectively |
698880 |
3.4.22.10 | W214A |
protease activity (microgram/min/mg) substrate azocasein: 0.8, substrate proSPE B C47S mutant: 0.8, compared to wild-type 341 and 378, respectively |
698880 |