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EC Number Crystallization (Commentary) Reference
Show all pathways known for 4.2.1.42Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.42sitting drop vapor diffusion method, using 2 M ammonium sulfate and 100 mM Bis-Tris-HCl (pH 5.5) 729259
Show all pathways known for 4.2.1.42Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.42structures of the wild type enzyme complexed with L-lyxarohydroxamate, and of the K197A mutant complexed with L-glucarate. Residue Lys 197 functions as the galactarate-specific base and His 328 functions as the L-talarate-specific base 678325
Show all pathways known for 4.2.1.42Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.42three different crystal forms are grown by hanging drop vapor diffusion at room temperature: (1) selenomethionine (SeMet)-substituted wild type enzyme, (2) wild-type enzyme complexed with Mg2+ and L-lyxarohydroxamate, and (3) the K197A mutant enzyme complexed with Mg2+ and L-glucarate 678325
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