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Literature summary for 4.2.1.42 extracted from

  • Groninger-Poe, F.P.; Bouvier, J.T.; Vetting, M.W.; Kalyanaraman, C.; Kumar, R.; Almo, S.C.; Jacobson, M.P.; Gerlt, J.A.
    Evolution of enzymatic activities in the enolase superfamily: galactarate dehydratase III from Agrobacterium tumefaciens C58 (2014), Biochemistry, 53, 4192-4203.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.42

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 2 M ammonium sulfate and 100 mM Bis-Tris-HCl (pH 5.5) Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 meso-galactarate at pH 7.9 and 22°C Agrobacterium tumefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
meso-galactarate Agrobacterium tumefaciens
-
 2-dehydro-3-deoxy-D-glucarate + H2O
-
 ?
meso-galactarate Agrobacterium tumefaciens C58 / ATCC 33970
-
 2-dehydro-3-deoxy-D-glucarate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens C58 / ATCC 33970
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, column chromatography, and gel filtration Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonate low level activity Agrobacterium tumefaciens 3-deoxy-D-xylo-hexarate + 3-deoxy-D-lyxo-hexarate + H2O
-
 ?
D-galacturonate low level activity Agrobacterium tumefaciens C58 / ATCC 33970 3-deoxy-D-xylo-hexarate + 3-deoxy-D-lyxo-hexarate + H2O
-
 ?
meso-galactarate
-
 Agrobacterium tumefaciens 2-dehydro-3-deoxy-D-glucarate + H2O
-
 ?
meso-galactarate
-
 Agrobacterium tumefaciens C58 / ATCC 33970 2-dehydro-3-deoxy-D-glucarate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
A9CG74
-
 Agrobacterium tumefaciens
galactarate dehydratase III
-
 Agrobacterium tumefaciens
GalrD-III
-
 Agrobacterium tumefaciens
m-galactarate dehydratase
-
 Agrobacterium tumefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 meso-galactarate at pH 7.9 and 22°C Agrobacterium tumefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5
-
 meso-galactarate at pH 7.9 and 22°C Agrobacterium tumefaciens