EC Number   |
Reference |
|---|
   4.2.1.115 | FlaA1-NADP+-UDP-GlcNAc and FlaA1-NADP+-UDPGlc ternary complexes, at room temperature by hanging-drop vapor diffusion in presence of NAD+, using as reservoir solution 10% v/v polyethylene glycol-200, 100 mM MES, pH 6.0, 5% v/w PEG 3000, and 4% acetone, and providing 10 mM excess of substrate, X-ray diffraction structure determination and analysis |
692980 |
| 4.2.1.115 | purified detagged enzyme expressed from Escherichia coli strain Rosetta(DE3), hanging drop vapour diffusion method, mixing of 0.001 ml of 6.5 mg/ml protein in 10 mM Tris, pH 8.0, with 500 nl of reservoir solution containing 0.1 M malic acid, and 22-25% PEG 3350 w/v, pH 7.0, at 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement using the structure of Helicobacter pylori enzyme FlaA1 (PDB entry 2GN4) as search model |
748176 |
| 4.2.1.115 | purified enzyme in complex with by-product UDP-xylo-sugar from 10 mM Tris-HCl. pH 9.0, 30 mM NaCl, and 1 mM DTT, suitable crystals are soaked in mother liquor supplemented with 25% v/v glycerol and 0.5 mlM UDP-D-GlcNAc, X-ray diffraction structure determination and analysis at 1.88 A resolution. The structure of CapE is virtually identical to that of apo-CapE in complex with a substrate analogue |
729735 |
