Crystallization (Comment) | Organism |
---|---|
FlaA1-NADP+-UDP-GlcNAc and FlaA1-NADP+-UDPGlc ternary complexes, at room temperature by hanging-drop vapor diffusion in presence of NAD+, using as reservoir solution 10% v/v polyethylene glycol-200, 100 mM MES, pH 6.0, 5% v/w PEG 3000, and 4% acetone, and providing 10 mM excess of substrate, X-ray diffraction structure determination and analysis | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
K133E | site-directed mutagenesis, inactive mutant | Helicobacter pylori |
K133M | site-directed mutagenesis, inactive mutant | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylglucosamine | Helicobacter pylori | the enzyme catalyzes the first step in the biosynthetic pathway of a pseudaminic acid derivative, which is implicated in protein glycosylation | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine = UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | reaction mechanism, FlaA1 is a UDP-GlcNAc 4,6-dehydratase that additionally inverts the chirality at the C-5 position | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylglucosamine | the enzyme catalyzes the first step in the biosynthetic pathway of a pseudaminic acid derivative, which is implicated in protein glycosylation | Helicobacter pylori | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | - |
? | |
UDP-N-acetylglucosamine | NMR reaction analysis, a possible three-step reaction mechanism that involves Lys133 functioning as both a catalytic acid and base | Helicobacter pylori | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | the enzyme possesses a hexameric doughnut-shaped quaternary structure, crystal stucture analysis | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
FlaA1 | - |
Helicobacter pylori |
inverting 4,6-dehydratase | - |
Helicobacter pylori |
More | the enzyme belongs to the SDR superfamily | Helicobacter pylori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | NADP+/NADPH is tightly bound to FlaA1 | Helicobacter pylori |