2.7.7.84 | OAS1, purified recombinant porcine OAS1, 1.5 mg/ml protein in 50 mM NaCl, 10 mM HEPES, pH 6.8, 0.5 mM EDTA, with 5 mM of iodoacetamide for 30 min at room temperature, then concentrated to 6 mg/ml for crystallization by vapor diffusion, best crystals grow at 20°C in 1:1 drops with a well solution of 30% PEG 2000 MME, 0.2 M ammonium sulfate, and 0.1 M sodium cacodylate at pH 6, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement |
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