EC Number |
Cofactor |
Reference |
---|
5.1.3.2 | more |
an NAD+ binding motif is GGXGXXG, not required for activity |
702141 |
5.1.3.2 | more |
contains an NAD+ binding site |
704314 |
5.1.3.2 | NAD+ |
- |
2320, 650515, 674580, 702074, 704314, 705584, 713793, 713965, 714374, 714602, 747889, 749149 |
5.1.3.2 | NAD+ |
1.78 mol of NAD+ per dimer. Each subunit is independently capable of being associated with one molecule of NAD+, suggestive of two NAD+ binding sites of epimerase per dimer |
661074 |
5.1.3.2 | NAD+ |
absolute requirement for NAD+. Km: 0.27 mM for the liver enzyme, Km: 0.28 mM for the mammary enzyme |
2317 |
5.1.3.2 | NAD+ |
associated to enzyme residue Lys150 |
749106 |
5.1.3.2 | NAD+ |
bound to the enzyme via GxxGxxG motif |
702074 |
5.1.3.2 | NAD+ |
bound within the active site cleft, binding structure, overview |
728628 |
5.1.3.2 | NAD+ |
coenzyme is tightly bound at the active site. NAD+ functions as the coenzyme for the interconversion of UDP-galactose and UDP-glucose by reversibly mediating their dehydrogenation to the common intermediate UDP-4-ketohexopyranoside. NAD+ activation induced by uridine nucleotides is brought about by a conformational change of epimerase that repositions Tyr149 at an increased distance from nicotinamide N1 of NAD+ while maintaining the electrostatic repulsion between Lys153 and nicotinamide N1 of NAD+ |
650023 |
5.1.3.2 | NAD+ |
contains firmly bound NAD+ |
2318, 2337 |