EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   3.4.21.B28 | 0.003 |
- |
7-methoxycoumarin-4-acetic acid-DRGETGP-dinitrophenyl |
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C |
678329 |
| 3.4.21.B28 | 0.003 |
- |
7-methoxycoumarin-4-acetic acid-DSGETGP-dinitrophenyl |
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C |
678329 |
| 3.4.21.B28 | 0.01 |
- |
Lys-Ala-7-amido-4-trifluoromethylcoumarin |
at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4 |
680739 |
| 3.4.21.B28 | 0.015 |
- |
7-methoxycoumarin-4-acetic acid-APGSKGDA-dinitrophenyl |
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C |
678329 |
| 3.4.21.B28 | 0.03 |
- |
Gly-Pro-7-amido-4-trifluoromethylcoumarin |
mutant enzyme A657D, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA |
678272 |
| 3.4.21.B28 | 0.05 |
- |
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin |
mutant enzyme E203A/E204A, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA |
678272 |
| 3.4.21.B28 | 0.059 |
- |
7-methoxycoumarin-4-acetic acid-DSGETGPA-dinitrophenyl |
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C |
678329 |
| 3.4.21.B28 | 0.067 |
- |
7-methoxycoumarin-4-acetic acid-DKGESGPA-dinitrophenyl |
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C |
678329 |
| 3.4.21.B28 | 0.079 |
- |
7-methoxycoumarin-4-acetic acid-ERGETGPAGG-dinitrophenyl |
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C |
678329 |
| 3.4.21.B28 | 0.081 |
- |
7-methoxycoumarin-4-acetic acid-DRGETGPA-dinitrophenyl |
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C |
678329 |
