EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
2.7.11.18 | -999 |
- |
more |
steady-state kinetics: comparison of smooth muscle myosin II and non-muscle myosin IIB as substrates, overview. MLCK has an about 2fold higher kcat for both smooth muscle myosin II substrates compared with non-muscle myosin IIB substrates, whereas Km values are very similar |
740266 |
2.7.11.18 | 5.17 |
- |
myosin light chain |
23-25°C, pH 7.5 |
640618 |
2.7.11.18 | 8 |
- |
[non-muscle heavy meromyosin IIB] |
pH 7.6, 25°C |
740266 |
2.7.11.18 | 12.6 |
- |
[non-muscle regulatory light chain] |
pH 7.6, 25°C |
740266 |
2.7.11.18 | 12.8 |
- |
[smooth muscle heavy meromyosin] |
pH 7.6, 25°C |
740266 |
2.7.11.18 | 16 |
- |
myosin light chain |
25°C, pH 7.6, isolated |
640615 |
2.7.11.18 | 19 |
- |
myosin light chain |
25°C, pH 7.6, bound to myosin |
640615 |
2.7.11.18 | 28.6 |
- |
[smooth muscle regulatory light chain] |
pH 7.6, 25°C |
740266 |
2.7.11.18 | 88 |
- |
myosin light chain |
25°C, pH 7.6, isolated, freshly prepared |
640615 |