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Literature summary for 2.7.11.18 extracted from
- Properties of myosin light chain kinase prepared from rabbit skeletal muscle by an improved method (1984), J. Biochem., 95, 1119-1130.
General Stability
| General Stability | Organism |
|---|---|
| unstable upon lyophilization | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Oryctolagus cuniculus |  |
| NaCl | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
myosin light chain | 25°C, pH 7.6, bound to myosin | Oryctolagus cuniculus | |
| 0.05 | 0.063 | isolated myosin light chain | 25°C, pH 7.6 | Oryctolagus cuniculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition of rabbit | Oryctolagus cuniculus |
| 94000 | - |
1 * 94000, SDS-PAGE | Oryctolagus cuniculus |
| 103000 | - |
sedimentation equilibrium method | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Oryctolagus cuniculus | - |
ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography on calmodulin-Sepharose | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24 | - |
skeletal muscle | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | - |
Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 94000, SDS-PAGE | Oryctolagus cuniculus |
| More | gel electrophoresis in various buffers gives different molecular weights | Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Oryctolagus cuniculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16 | - |
myosin light chain | 25°C, pH 7.6, isolated | Oryctolagus cuniculus | |
| 19 | - |
myosin light chain | 25°C, pH 7.6, bound to myosin | Oryctolagus cuniculus | |
| 88 | - |
myosin light chain | 25°C, pH 7.6, isolated, freshly prepared | Oryctolagus cuniculus | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 9.2 | about half-maximal activity at pH 6.3 and 9.2 | Oryctolagus cuniculus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
rapid inactivation below | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | requirement | Oryctolagus cuniculus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Oryctolagus cuniculus | isoelectric focusing, pH 3-10, 4% polyacrylamide gel or flat bed isoelectric focusing with Sephadex G-200 | - |
5 |
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