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EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Reference
1.2.1.27	-999	-	more	Kcat-value of CoA for R301L mutant enzyme under steady-state conditions with propionaldehyde is above 3 1/sec at pH 8.2 and 30°C	725429
1.2.1.27	0.003	-	2-Methyl-3-oxopropanoate	pH 8.2, 30°C	722211
1.2.1.27	0.003	-	methylmalonate semialdehyde	30°C, pH 8.2	722211
1.2.1.27	0.02	-	malonate semialdehyde	30°C, pH 8.2	722211
1.2.1.27	0.02	-	malonate semialdehyde	pH 8.2, 30°C	722211
1.2.1.27	0.02	-	NAD+	V229G/H226P/G225 insertion, pH 8.2 and 30°C	725485
1.2.1.27	0.02	-	NAD+	V229G/Y252L/V253I/G225 insertion, pH 8.2 and 30°C	725485
1.2.1.27	0.03	-	propionaldehyde	30°C, pH 8.2	722211
1.2.1.27	0.03	-	propionaldehyde	pH 8.2, 30°C	722211
1.2.1.27	0.06	-	NAD+	R124L mutant enzyme, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C	725429

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Release 2023.1 (January 2023)