EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference  |
|---|
    6.1.1.9 | 2.9 |
- |
valine |
- |
545 |
| 6.1.1.9 | 5.65 |
- |
valine |
enzyme expressed in E. coli |
545 |
| 6.1.1.9 | -999 |
- |
more |
rate of hydrolysis of Thr-tRNAVal and Val-tRNAVal by wild-type and mutant enzyme, incorporation of amino acids valine and threonine into the enzyme |
650122 |
| 6.1.1.9 | 0.064 |
- |
L-valine |
aminoacylation reaction, His-tagged K277A mutant enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 1.5 |
- |
L-valine |
aminoacylation reaction, His-tagged wild-type enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 1.5 |
- |
tRNAVal |
aminoacylation reaction with L-valine, K277A mutant enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 1.7 |
- |
tRNAVal |
aminoacylation reaction with L-valine, wild-type enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 50 |
- |
ATP |
ATP-diphosphate exchange reaction, His-tagged wild-type enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 50 |
- |
tRNAVal |
ATP-diphosphate exchange reaction, K277A mutant enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 51 |
- |
tRNAVal |
ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.5, 25°C |
650122 |
