EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate  |
Reference |
|---|
    6.1.1.9 | 0.28 |
- |
ATP |
pH 7.5, 37°C, mutant D286A, in presence of tRNA |
715519 |
| 6.1.1.9 | 0.34 |
- |
ATP |
pH 7.5, 37°C, mutant K277P, in presence of tRNA |
715519 |
| 6.1.1.9 | 0.48 |
- |
ATP |
pH 7.5, 37°C, mutant K277P/D286A, in presence of tRNA |
715519 |
| 6.1.1.9 | 12.9 |
- |
ATP |
pH 7.5, 37°C, wild-type ValRS, in presence of tRNA |
715519 |
| 6.1.1.9 | 50 |
- |
ATP |
ATP-diphosphate exchange reaction, His-tagged wild-type enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 57 |
- |
ATP |
ATP-diphosphate exchange reaction, His-tagged K277A mutant enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 0.064 |
- |
L-valine |
aminoacylation reaction, His-tagged K277A mutant enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 0.074 |
- |
L-valine |
at pH and °C |
727894 |
| 6.1.1.9 | 0.68 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C |
653865 |
| 6.1.1.9 | 0.99 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C |
653865 |
