EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    6.1.1.9 | 0.68 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C |
653865 |
| 6.1.1.9 | 0.99 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C |
653865 |
| 6.1.1.9 | 1.06 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal inosine base analogue, pH 7.5, 37°C |
653865 |
| 6.1.1.9 | 1.5 |
- |
L-valine |
aminoacylation reaction, His-tagged wild-type enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 1.5 |
- |
tRNAVal |
aminoacylation reaction with L-valine, K277A mutant enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 1.7 |
- |
tRNAVal |
aminoacylation reaction with L-valine, wild-type enzyme, pH 7.5, 25°C |
650122 |
| 6.1.1.9 | 2.9 |
- |
valine |
- |
545 |
| 6.1.1.9 | 4.3 |
- |
tRNAVal |
R818A/R843A mutant enzyme, pH 7.7, 65°C |
653871 |
| 6.1.1.9 | 5.65 |
- |
valine |
enzyme expressed in E. coli |
545 |
| 6.1.1.9 | 6.08 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C |
653865 |
