EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
6.1.1.9 | 6.08 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal inosine base analogue, pH 7.5, 37°C |
653865 |
6.1.1.9 | 6.08 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C |
653865 |
6.1.1.9 | 6.7 |
- |
tRNAVal |
wild-type enzyme, pH 7.7, 65°C |
653871 |
6.1.1.9 | 12.9 |
- |
ATP |
pH 7.5, 37°C, wild-type ValRS, in presence of tRNA |
715519 |
6.1.1.9 | 13.9 |
- |
L-valine |
with wild-type tRNAVal, pH 7.5, 37°C |
653865 |
6.1.1.9 | 14.4 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal purine riboside base analogue, pH 7.5, 37°C |
653865 |
6.1.1.9 | 50 |
- |
ATP |
ATP-diphosphate exchange reaction, His-tagged wild-type enzyme, pH 7.5, 25°C |
650122 |
6.1.1.9 | 50 |
- |
tRNAVal |
ATP-diphosphate exchange reaction, K277A mutant enzyme, pH 7.5, 25°C |
650122 |
6.1.1.9 | 51 |
- |
tRNAVal |
ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.5, 25°C |
650122 |
6.1.1.9 | 57 |
- |
ATP |
ATP-diphosphate exchange reaction, His-tagged K277A mutant enzyme, pH 7.5, 25°C |
650122 |